pQTEV-KCNIP2 (BioVector-034799) BioVector NTCC质粒载体菌种细胞基因保藏中心

pQTEV-KCNIP2 (BioVector-034799) BioVector NTCC质粒载体菌种细胞基因保藏中心 Vector backbone pQTEV (Search Vector Database) Backbone size w/o insert (bp) 4700 Vector type Bacterial Expression GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) Rosetta Copy number Unknown GENE/INSERT Gene/Insert name Kv channel interacting protein 2 Alt name KCNIP2 Alt name PSFEp250G064 Alt name DKFZp566L1246 Species H. sapiens (human) GenBank IDDQ000562 Entrez Gene KCNIP2 (a.k.a. KCHIP2) Tags / Fusion Proteins His (N terminal on backbone) TEV (N terminal on backbone) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site BamHI (not destroyed) 3′ cloning site NotI (not destroyed) 5′ sequencing primer TGAGCGGATAACAATTTCACACAG 3′ sequencing primer GGCAACCGAGCGTTCTGAAC
The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
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