DOHH2 cell line BioVector NTCC质粒载体菌种细胞基因保藏中心

DOHH2 cell line BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line: DOHH-2 DSMZ no.: ACC 47Species: human (Homo sapiens)Cell type: B cell lymphoma Origin: established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described to carry the t(14;18) leading to IGH@-BCL2 (IGH-BCL2) fusion gene and to express BCL2 mRNA; the original culture was a mixture of EBV - and EBV + cells, of which the EBV - cells were isolated and clonally expanded Reference(s): 14671Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:cells grow as single cells or in clumps in suspension; image; image; imageMedium: 90% RPMI 1640 + 10% h.i. FBS Subculture: maintain at 0.3-0.7 x 106 cells/ml; split 1:2 every second day; seed out at ca. 0.5 x 106 cells/ml; after thawing, culture cells temporarily in 24-well plates Incubation: at 37 °C with 5% CO2 Doubling time: ca. 40 hours Harvest: maximal density of about 0.7-1.0 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of AST, MDH, NP Cytogenetics: human hyperdiploid karyotype - 47(43-48)<2n>XY, +7, der(8)t(8;18)(q24;q21), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - earliest of a recent series of cell lines with cohabiting 8;14 (Burkitt or diffuse large cell lymphoma) and 14;18 (follicular lymphoma) translocations Molec. Genetics: expression of fusion gene IGH@-BCL2 (IGH-BCL2) confirmed by RT-PCR Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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