pSPV-EGFP重组转移载体Orf病毒荧光表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSPV-EGFP重组转移载体Orf病毒荧光表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心 the pSPV-EGFP vector provides a direct, fast, and convenient way to manipulate the recombinant viruses. a rapid method to generate recombinant Orf viruses (ORFV) based on the enhanced green fluorescent protein (EGFP) reporter gene as a selectable marker. The coding sequence of EGFP gene was amplified from pEGFP-N1 vector and subcloned into the pZIPPY-neo/gus plasmid under the control of the early-late vaccinia virus (VACV) VV7.5 promoter and flanked by two multiple cloning sites (MCS) to generate a novel transfer vector pSPV-EGFP. pSPV-EGFP重组转移载体Orf病毒荧光表达质粒
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