Universal Mycoplasma Detection Kit (ATCC® 30-1012K™) BioVector NTCC质粒载体菌种细胞基因保藏中心

Universal Mycoplasma Detection Kit (ATCC® 30-1012K™) BioVector NTCC质粒载体菌种细胞基因保藏中心 ATCC® Number: 30-1012K™ Applications: The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity. The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel. Component Volume Composition Storage Lysis Buffer 2 mL Lytic agent + digestive enzymes -20°C Universal PCM Mix 0.8 mL Proprietary mix of buffers, dNTPs, thermostable polymerase -20°C Universal PCR Primers 0.1 mL Proprietary mix of universal forward and reverse primers -20°C Sample Lysis Tubes 40 each 2 mL snap cap tubes, tight seal -20°C Positive Control 50 µL 1 pg/µL pUC19::M. arginini target in TE -20°C
FAQs Universal Mycoplasma Detection Kit - Differences from former Mycoplasma Detection Kit The... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Sensitivity The kit detects less than 20 genomes of Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Species Detected Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Time to Obtain Results Total time from sample preparation to results is less than 3 hours. Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Identifying Contaminant Species A positive mycoplasma results in a specific band following agarose gel electrophoresis. If speciation is required, the band can be excised from the gel and sequenced. This will identify the ... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Species Recognized The assay detects over 60 unique strains of mycoplasma including the eight species most likely to afflict cell cultures: Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Testing Cell Culture Supernatant Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Testing Media The... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Testing Adherent or Suspension Cells Either adherent or suspension cells can be tested. Adherent cells should be harvested at 50-70% confluency and suspension cells should be harvested in log-phase growth. Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Antibiotics in Medium Alt... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Storing Samples from Interrupted Procedure Universal Mycoplasma Detection Kit - Taq DNA Polymerase Universal Mycoplasma Detection Kit - PCR for Mycoplasma Detection PCR... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Primers Universal forward and reverse primers are a component of the kit. The primers target the highly conserved 16S rRNA region of the mycoplasma genome and generate a specific PC... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Reaction Tubes Universal Mycoplasma Detection Kit - Not Using Touchdown PCR If you use regular PCR instead of the touchdown procedure, non-specific amplification may occur and bands may be present th... Date Updated: 3/27/2014 Universal Mycoplasma Detection Kit - Touchdown Protocol Universal Mycoplasma Detection Kit - Controls A positive control (pUC19::M. arginini target) is included to verify that the PCR reaction was successful. A negative control is prepared by combining the Universal... Date Updated: 3/27/2014 Universal Mycplasma Detection Kit - Skipping Inactivation Step The assay will NOT work. The protease in the Lysis Mix must be inactivated prior to adding the lysate to the PCR mixture. Date Updated: 3/27/2014
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