pBabepuro-myc-ER (BioVector-019128) BioVector NTCC质粒载体菌种细胞基因保藏中心

pBabepuro-myc-ER (BioVector-019128) BioVector NTCC质粒载体菌种细胞基因保藏中心 19128 pBabepuro-myc-ER AmpR.1Stab culture,in E.coli. Storage:4℃ Recovery 1. Obtain an LB agar plate with the appropriate antibiotic. 2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.) 3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1. 4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2. 5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3. 6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet). 7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies. • Vector backbone pBabepuro3:hbER tam • Backbone size w/o insert (bp)5169 • Vector type Mammalian Expression, Retroviral • Selectable markers Puromycin GROWTH IN BACTERIA • Bacterial Resistance(s) Ampicillin • Growth Temperature 37°C • Growth Strain(s) DH5alpha • Copy number High Copy GENE/INSERT • Gene/Insert name c-myc • Species H. sapiens (human) • Entrez Gene MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc) • Tag / Fusion Protein • mouse ER hormone binding domain (C terminal on insert) CLONING INFORMATION • Cloning methodUnknown • 5′ sequencing primerpBABE-5 • 3′ sequencing primerpBABE-3 Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam).
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