Flavobacterium johnsoniae ATCC 17061 BioVector NTCC质粒载体菌种细胞基因保藏中心

Flavobacterium johnsoniae ATCC 17061 BioVector NTCC质粒载体菌种细胞基因保藏中心 Designation: MYX.1.1.1 [DSM 2064, LMG 1341, NCIB 11054] Deposited Name: Cytophaga johnsonae Stanier Medium ATCC® Medium 112: Van Niel's yeast agar Growth Conditions Temperature: 30°C Atmosphere: Aerobic Propagation Procedure 1. Open vial according to enclosed instructions. 2. From a single tube of #112 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet. 3. Aseptically transfer this aliquot back into the broth tube. Mix well. 4. Use several drops of the suspension to inoculate a second tube of broth, a slant and/or plate. 5. Incubate all tubes and plate at 30°C for 24 to 48 hours.
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