HCT116/L cell line人结肠癌耐奥沙利铂耐药株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HCT116/L cell line人结肠癌耐奥沙利铂耐药株 BioVector NTCC质粒载体菌种细胞基因保藏中心 HCT116/L cell line Cat No.: NTCC595981 Oxaliplatin (L-OHP)-resistant colorectal cancer cells HCT116/L-OHP. Organism Homo sapiens, human Tissue colon Morphology epithelial Culture Properties adherent Disease colorectal carcinoma Age adult Gender male Applications This cell line is a suitable transfection host. This line has a mutation in codon 13 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon. Karyotype The stemline chromosome number is near diploid with the modal number at 45 (62%) and polyploids occurring at 6.8%. The markers 10q+ and t(?8p;18q) are present in all metaphases and t(9q;?16p-), in 80% of the cells karyotyped. N16 is monosomic in the presence of, but disomic in the absence of t(9q;?16p-). N10 and N18 are monosomic and other chromosomes from those mentioned above are disomic. Q-band observations revealed the presence of the Y chromosome, but not in all cells (50% of cells lacked the Y in G-band karyotypes). Images Clinical Data male Genes Expressed carcinoembryonic antigen (CEA) 1 ng per 106 cells per 10 days. Cellular Products carcinoembryonic antigen (CEA) 1 ng per 10 exp6 cells per 10 days; keratin Tumorigenic Yes Effects Yes, in nude mice Comments The cells are positive for keratin by immunoperoxidase staining. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression. Complete Growth Medium The base medium for this cell line is McCoy's 5a Medium Modified. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes are given for a 75 cm2 flask. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C Growth Conditions: Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts. STR Profile Amelogenin: X,Y CSF1PO: 7,10 D13S317: 10,12 D16S539: 11,13 D5S818: 10,11 D7S820: 11,12 THO1: 8,9 TPOX: 8,9 vWA: 17,22 Isoenzymes AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1 References Schroy PC, et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092 Brattain MG, et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343
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