GFP-nucleolin BioVector NTCC质粒载体菌种细胞基因保藏中心

GFP-nucleolin BioVector NTCC质粒载体菌种细胞基因保藏中心 There are a few mutations (K132R, K141E, & K142E and G680S) in the nucleolin but they are not thought to affect function.
Vector backbone pEGFPC1 (Search Vector Database) Backbone manufacturer Clontech Backbone size w/o insert (bp) 4700 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA Bacterial Resistance(s) Kanamycin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name Nucleolin Species H. sapiens (human) Insert Size (bp) 2100 Entrez Gene NCL (a.k.a. C23) Tag / Fusion Protein GFP (N terminal on backbone) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site KpnI (not destroyed) 3′ cloning site BamHI (not destroyed) 5′ sequencing primer EGFP-C 3′ sequencing primer SV40-pA-R [Supplier来源] http://www.biovector.net