Hepa 1-6 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Hepa 1-6 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Hepa 1-6 cell line Cat No.: NTCC595981 Culture Type: Cell line Cell Line Name: Hepa 1-6 Other Collection No.: ATCC CRL 1830 Keywords: Mouse hepatoma Cell Line Description: Derived from BW7756 tumour in a C57L mouse. Secretes several liver products including albumin, alpha-fetoprotein, alpha 1-antitrypin and amylase. Species: Mouse Tissue of Origin: liver CellType: Epithelial-like Growth Mode: Adherent Karyotype: Not specified Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Country: USA References: Darlington GJ. 1987 Liver cell lines. Methods Enzymol.;151:19-38. PMID: 3431441. Additional Bibliography: Darlington GJ, Bernhard HP, Miller RA, Ruddle FH. 1980 Expression of liver phenotypes in cultured mouse hepatoma cells. J Natl Cancer Inst. 64(4):809-19. PMID: 6102619. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net