Bacillus sphaericus BioVector NTCC质粒载体菌种细胞基因保藏中心

Bacillus sphaericus BioVector NTCC质粒载体菌种细胞基因保藏中心 Deposited As Bacillus sphaericus Meyer and Neide Strain Designations [1013, CCM 2120, NCTC 10338] Biosafety Level 1 Type Strain yes Comments Reference strain for DNA similarity group I Medium Nutrient agar or nutrient broth Nutrient Agar/Broth Agar Medium Nutrient Agar (BD 213000)…………………..23 g DI Water………………………………….……1000 ml Autoclave at 121ºC. Broth Medium Nutrient Broth (BD cat 234000)…………….8.0 g DI Water…………………………………..….1000 ml Autoclave at 121ºC. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Nutrient Agar Composition Beef Extract……………………..…………..3.0 g Peptone……..…………………….…………5.0 g Agar………………………………………….15.0 g Final pH 6.8 +/- 0.2. *Omit agar for broth medium Growth Conditions Temperature: 30.0°C References J. Gen. Microbiol. 34: 270, 1964. Int. Bull. Bacteriol. Nomencl. Taxon. 12: 75, 1962. Meyer A, Neide E. Botanische beschreibung einiger sporenbildenden bakterien. Zentralbl. Bakteriol., II Abt. 12: 337-352, 1904. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net