KG-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

KG-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 KG-1 cell line Cat No.: NTCC596014 Organism Homo sapiens, human Tissue bone marrow Cell Type Macrophage Product Format frozen Morphology myeloblast Culture Properties suspension Biosafety Level 1 Disease acute myelogenous leukemia Age 59 years Gender male Ethnicity Caucasian Applications This cell line is a suitable transfection host. Karyotype The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations. Derivation The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. Clinical Data 59 years Caucasian male Antigen Expression HLA A30, A31, B35, Cw4 Genes Expressed HLA DR Cellular Products HLA DR Comments KG-1 myeloblasts can differentiate into macrophages in the presence of phorbol esters. They show a good response to colony stimulating factor (CSF). The line is EBNA negative (EBNA-). Complete Growth Medium The base medium for this cell line is Iscove's Modified Dulbecco's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL. Do not allow to exceed 2 x 106 cells/mL. Medium Renewal: Twice per week Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C STR Profile Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 Isoenzymes AK-1, 0 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 0 References Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net