HHV8 Human herpesvirus 8 virus病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心

HHV8 Human herpesvirus 8 virus病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心 Classification Herpesviridae, Rhadinovirus Agent Human herpesvirus 8 Strain L1T2 Cell Line Common Name Kaposi’s sarcoma-associated herpes virus Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Product Format frozen 1 mL per vial Storage Conditions Vapor phase of liquid nitrogen
Comments General observation is that it may take four passages of the cells until the percentage of LANA positive cells reach ≥ 50% and stabilize. Effect on Host Non-cytopathic, visualized by fluorescent staining with LANA specific conjugated antibodies
Growth Conditions Temperature: 37°C Recommendations for Infection: Pellet cells gently (e.g. 10 minutes at 200 x g at 20°C) in medium to remove DMSO. Re-suspend pellet in fresh medium. Passage cells by transferring into a flask containing additional medium. Incubate flask at 37°C, 5% CO2 with humidity until cells reach 80-90% confluence. Harvest cells by trypsinization with 0.25% Trypsin (ATCC® 30-2101™). Continue to passage product until at least ≥ 50% of cells are immunofluorescence positive. Replace a portion (e.g. 75%) of medium every 2-3 days. Incubation: 1-7 days
Effect on Host Non-cytopathic, visualized by fluorescent staining with LANA specific conjugated antibodies
Source Cell line created by infection of human primary endothelial cells References An FQ, et al. Long-term-infected telomerase-immortalized endothelial cells: a model for Kaposi's sarcoma-associated herpesvirus latency in vitro and in vivo. J. Virol. 80(10): 4833-4846, 2006. PubMed: 16641275
[Supplier来源] http://www.biovector.net