HHV6 Human herpesvirus 6 virus病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心

HHV6 Human herpesvirus 6 virus病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心 Human herpesvirus 6
Classification Herpesviridae, Roseolovirus Deposited As Human herpesvirus 6 Agent Human herpesvirus 6B Strain SF Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Product Format frozen 1 mL per vial Storage Conditions Vapor phase of liquid nitrogen
Effect on Host CPE, cell enlargement and balloon-like degeneration
Recommended Host Human peripheral blood mononuclear cells Growth Conditions Temperature: 37°C Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 5 x 105 cells per mL. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate at 37°C in a humidified 5% CO2 atmosphere. Incubation: 7-9 days
Special Collection NCRR Contract Source Saliva from an HIV-infected patient, San Francisco, California References Levy JA, et al. Characterization of a new strain of HHV-6 (HHV-6SF) recovered from the saliva of an HIV-infected individual. Virology 178: 113-121, 1990. PubMed: 1975142 Jay A Levy, personal communication
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