Escherichia coli GFP荧光大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Escherichia coli GFP荧光大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Biovector692782 Escherichia coli GFP 1Vial,in E.coli. AmpR.Storage:4℃ Escherichia coli GFP Application Fluorescence detection Fluorescence imaging FACS Cell infection assays In vivo localization Pathogen-host interactions assays Biosafety Level 1 Antigenic Properties Serotype 06, Biotype 1 Type Strain no Antigenic Properties Serotype 06, Biotype 1 Antibiotic Resistance Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 100 µg/mL ampicillin. Comments This clone was derived from 25922 and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Placpromoter. This construct has been designed for Gram-negative bacteria fluorescence labeling. Vector: pUCP18-MCSgfpmut3 Vector size: 5.492kb Vector type: plasmid Marker: bla (ampR) Features: GFPmut3: fluorescent maker under the control of Plac promoter Origin of replication: ori from pRO1600 Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI. Double restriction sites: HindIII, PstI, SalI, XbaI. Medium Tryptic Soy Agar/Broth with 100 mcg/ml Ampicillin Growth Conditions Temperature: 37°C Atmosphere: Aerobic References Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227 [Supplier来源] http://www.biovector.net