E.coli DY380,SW102,SW105,SW106, EL250,EL350 strains BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19752
- 货 号：E.coli DY380,SW102,SW105,SW106, EL250,EL350 strains
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
E.coli DY380,SW102,SW105,SW106, EL250,EL350 strains BioVector NTCC质粒载体菌种细胞基因保藏中心 DY380 : The original, DH10B-derived strain. These bacteria contain a defective λ prophage with recombination proteins exo, bet, and gam being controlled by the temperature-sensitive repressor cI857. The bacteria are tet resistant (12.5 μg/ml). Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) <> tet]
Reference
EL250 : Derived from DY380. In addition to the recombination genes found in DY380 EL250 also contains a tightly controlled arabinose-inducible flpe gene. flpe will mediate recombination between two identical frt sites. This strain is not tet resistant. Genotype: DY380 [(cro-bioA) <> araC-PBADflpe]
Reference
EL350 : Derived from DY380. In addition to the recombination genes found in DY380 EL350 also contains a tightly controlled arabinose-inducible cre gene. cre will mediate recombination between two identical loxP sites. This strain is not tet resistant. Genotype: DY380 [(cro-bioA) <> araC-PBADcre]
Reference
SW102: Derived from DY380 and has all the same features, but designed for BAC recombineering using galK positive/negative selection. These bacteria contain a fully functional gal operon, except the galK gene is missing. The ability to grow on galactose minimal medium can be restored by adding galK in trans, by inserting a galK expression cassette into a BAC. Next, the galK cassette can be replaced by any dsDNA carrying any desired mutation, by selection against galK using 2-deoxy-galactose.
Reference
SW105: Derived from EL250. Like SW102, these cells can be used for galK positive/negative selection. Like EL250, these cells contain an ara-inducible Flpe gene.
Reference
SW106: Derived from EL350. Like SW102, these cells can be used for galK positive/negative selection. Like EL350, these cells contain an ara-inducible Cre gene.
Reference
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 www.biovector.net [Supplier来源] http://www.biovector.net
Reference
EL250 : Derived from DY380. In addition to the recombination genes found in DY380 EL250 also contains a tightly controlled arabinose-inducible flpe gene. flpe will mediate recombination between two identical frt sites. This strain is not tet resistant. Genotype: DY380 [(cro-bioA) <> araC-PBADflpe]
Reference
EL350 : Derived from DY380. In addition to the recombination genes found in DY380 EL350 also contains a tightly controlled arabinose-inducible cre gene. cre will mediate recombination between two identical loxP sites. This strain is not tet resistant. Genotype: DY380 [(cro-bioA) <> araC-PBADcre]
Reference
SW102: Derived from DY380 and has all the same features, but designed for BAC recombineering using galK positive/negative selection. These bacteria contain a fully functional gal operon, except the galK gene is missing. The ability to grow on galactose minimal medium can be restored by adding galK in trans, by inserting a galK expression cassette into a BAC. Next, the galK cassette can be replaced by any dsDNA carrying any desired mutation, by selection against galK using 2-deoxy-galactose.
Reference
SW105: Derived from EL250. Like SW102, these cells can be used for galK positive/negative selection. Like EL250, these cells contain an ara-inducible Flpe gene.
Reference
SW106: Derived from EL350. Like SW102, these cells can be used for galK positive/negative selection. Like EL350, these cells contain an ara-inducible Cre gene.
Reference
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 www.biovector.net [Supplier来源] http://www.biovector.net
- 公告/新闻
