pTet-O-Ngn2-puro plasmid 52047 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTet-O-Ngn2-puro plasmid 52047 BioVector NTCC质粒载体菌种细胞基因保藏中心 Expresses Ngn2 and puromycin resistent gene under control of TetON promoter
Vector backbone Tet-O-FUW
(Search Vector Database)
Backbone size w/o insert (bp) 7745
Total vector size (bp) 9861
Vector type Mammalian Expression, Lentiviral
Selectable markers Puromycin
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number High Copy
GENE/INSERT

Gene/Insert name Ngn2
Species M. musculus (mouse)
Insert Size (bp) 1071
GenBank IDNM_009718.2
Entrez Gene Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)
Promoter tet-on
Tag / Fusion Protein
T2A-and puromycin resistant cassette (C terminal on insert)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site EcoRI (not destroyed)
3′ cloning site XbaI (not destroyed)
5′ sequencing primer LNCX
3′ sequencing primer WPRE-R
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 www.biovector.net [Supplier来源] http://www.biovector.net