iDuet011a
(BioVector-017628) BioVector NTCC质粒载体菌种细胞基因保藏中心

iDuet011a
(BioVector-017628) BioVector NTCC质粒载体菌种细胞基因保藏中心 Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).

Tet-Inducible Vector. The 309bp tetO7 sequence was PCR-amplified from the template pLVTH-siGFP. The inducible iDuet011A vector has the tetO7 sequence inserted at the PacI site (with a BstXI linker), immediately upstream of the UBC promoter
Vector backbone Duet011
(Search Vector Database)
Vector type Mammalian Expression, Lentiviral
Selectable markers Hygromycin ; GFP
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number High Copy
GENE/INSERT

Gene/Insert name TetO7 - UBC promoter - GFP - PGK promoter - Hygro
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ sequencing primer EGFP-N
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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