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Taq聚合酶高表达质粒载体菌种-BioVector NTCC保藏中心

  • 价　　格：¥4920
  • 货　　号：Taq DNA polymerase expression plasmid
  • 产　　地：北京
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Taq DNA polymerase聚合酶高表达质粒载体菌种



Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

Highlights

  • Isolated from a recombinant source

  • Supplied with 10X Reaction Buffer

  • Robust and reliable reactions

  • Tolerates a wide range of templates

  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides

  • Exceptional value in terms of cost per unit

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Reagents Supplied

The following reagents are supplied with this product:


Store at (°C)Concentration
Standard Taq Reaction Buffer Pack-2010X

Advantages and FeaturesBack to Top

Features

  • Industry standard for routine PCR

Applications

  • PCR

  • Primer Extension

  • DHPLC

  • Microarray Analysis

  • Colony PCR

Properties and UsageBack to Top

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Standard Taq Reaction Buffer Pack

1XStandard Taq Reaction Buffer Pack:
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
pH 8.3 @ 25°C

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 94000 daltons

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

No

Strand Displacement

+1

Resulting Ends

  • Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA

Error Rate

~ 285x10-6bases

NotesBack to Top

  1. 5'→3' flap endonuclease degrades displaced strand.

ReferencesBack to Top

  1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.

  2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.

  3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.

  4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.

  5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.

  6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.

  7. Powell, L.M. et al. (1987). Cell. 50, 831-840.

  8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.

  9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.


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