pgRNA-bacteria细菌CRISPR/Cas9载体质粒，表达guide RNA，用于细菌基因敲除-BioVector NTCC保藏中心

价　　格：¥2930
货　　号：pgRNA-bacteria
产　　地：北京

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pgRNA-bacteria
(BioVector-044251)

细菌CRISPR/Cas9载体质粒，表达guide RNA，用于细菌基因敲除。

配套质粒：

pdCas9-bacteria
pwtCas9-bacteria
pCRISPR
pCas9
pdCas9

pgRNA-bacteria

pgRNA-bacteria map

PURPOSE
Expression of customizable guide RNA (gRNA) for bacterial gene knockdown
DEPOSITING LAB
Stanley Qi
PUBLICATION
Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.

BACKBONE

Vector backbone
pUC19
(Search Vector Database)
Vector type
Bacterial Expression, CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
Top10
Copy number
High Copy

GENE/INSERT

Gene/Insert name
guide RNA (bacteria)
Insert Size (bp)
102
PromoterBBa_J23119 (SpeI)

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ cloning siteSpeI (not destroyed)
3′ cloning siteHindIII (not destroyed)
5′ sequencing primern/a
3′ sequencing primern/a

SEQUENCE INFORMATION

>Contains bacteria guide RNA expression

gAATTCTAAAGATCTTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTAACTTTCAGTTTAGCGGTCT

GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGT

CGGTGCTTTTTTTGAAGCTTGGGCCCGAACAAAAACTCatctcagaagaggatctgaatagcgccgtcga

ccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattt

tcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtag

cgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtgggg

tctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactGGGCC

TTTCGTTTTATCTGTTGTTTGTCGGTGAACTGGATCCTTACTCGAGTCTAGACTGCAGgcttcctcgctc

actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt

tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg

taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgc

tcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg

tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc

gctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg

cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa

gacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgc

tacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctg

ctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg

gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt

ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa

aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaa

cttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatc

catagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgct

gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg

ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctag

agtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgc

tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgt

tgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatc

actcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgact

ggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaa

tacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcg

aaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatct

tcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagg

gaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatca

gggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgc

acatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaata

ggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctg

For your References section:
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860

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