pwtCas9-bacteria细菌CRISPR/Cas9载体质粒，含有野生型Cas9元件，TetO四环素诱导调控-BioVector NTCC保藏中心

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货　　号：pwtCas9-bacteria
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pwtCas9-bacteria
(BioVector-044250)

细菌CRISPR/Cas9载体质粒，含有野生型Cas9 (S .pyogenes)，TetO诱导表达调控。

配套质粒：

pgRNA-bacteria
pdCas9-bacteria

BACKBONE

Vector backbone
pUC19
(Search Vector Database)
Vector type
Bacterial Expression, CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
Top10
Copy number
High Copy

PURPOSE
aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown
DEPOSITING LAB
Stanley Qi
PUBLICATION
Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.

pwtcas9-bacteria

GENE/INSERT

Gene/Insert name
wild-type Cas9
Species
S .pyogenes
Insert Size (bp)
4107
PromoterpLtetO-1

CLONING INFORMATION

Cloning methodRestriction Enzyme
5′ cloning siteBglII (not destroyed)
3′ cloning siteXhoI (not destroyed)
5′ sequencing primeragaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGATAAGAAATACTCAATAGG
3′ sequencing primerggagatccttactcgagttaGTCACCTCCTAGCTGACTCAAATCAAT

SEQUENCE INFORMATION

>wild-type Cas9 (S. pyogenes)

GCATGCTTAAGACCCACTTTCACATTTAAGTTGTTTTTCTaatccgcatatgatcaattcaaggccgaat

aagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactat

cagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagt

aaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggct

aattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgc

gatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttc

taaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgctta

ttttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaac

cttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctagacat

cattaattcctaatttttgttgacactctatcgttgatagagttattttaccactccctatcagtgatag

agaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGATAAGAAATACTCAATAGGCTTAGATA

TCGGCACAAATAGCGTCGGATGGGCGGTGATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAAGGT

TCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGGGCTCTTTTATTTGACAGTGGAGAG

ACAGCGGAAGCGACTCGTCTCAAACGGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGTT

ATCTACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTTCATCGACTTGAAGAGTC

TTTTTTGGTGGAAGAAGACAAGAAGCATGAACGTCATCCTATTTTTGGAAATATAGTAGATGAAGTTGCT

TATCATGAGAAATATCCAACTATCTATCATCTGCGAAAAAAATTGGTAGATTCTACTGATAAAGCGGATT

TGCGCTTAATCTATTTGGCCTTAGCGCATATGATTAAGTTTCGTGGTCATTTTTTGATTGAGGGAGATTT

AAATCCTGATAATAGTGATGTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTTGAA

GAAAACCCTATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTTCTGCACGATTGAGTAAATCAAGAC

GATTAGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAAAAATGGCTTATTTGGGAATCTCATTGCTTT

GTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATGCTAAATTACAGCTTTCA

AAAGATACTTACGATGATGATTTAGATAATTTATTGGCGCAAATTGGAGATCAATATGCTGATTTGTTTT

TGGCAGCTAAGAATTTATCAGATGCTATTTTACTTTCAGATATCCTAAGAGTAAATACTGAAATAACTAA

GGCTCCCCTATCAGCTTCAATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTTTAAAAGCT

TTAGTTCGACAACAACTTCCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATGCAG

GTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTTTAGAAAAAATGGA

TGGTACTGAGGAATTATTGGTGAAACTAAATCGTGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAAC

GGCTCTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAGACAAGAAGACTTTTATC

CATTTTTAAAAGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCC

ATTGGCGCGTGGCAATAGTCGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTACCCCATGGAAT

TTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAAA

ATCTTCCAAATGAAAAAGTACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTTTATAACGAATT

GACAAAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTTCTTTCAGGTGAACAGAAGAAAGCC

ATTGTTGATTTACTCTTCAAAACAAATCGAAAAGTAACCGTTAAGCAATTAAAAGAAGATTATTTCAAAA

AAATAGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGAAGATAGATTTAATGCTTCATTAGGTACCTA

CCATGATTTGCTAAAAATTATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAG

GATATTGTTTTAACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATATGCTC

ACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTCGCCGTTATACTGGTTGGGGACGTTTGTCTCG

AAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATATTAGATTTTTTGAAATCAGATGGT

TTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGATAGTTTGACATTTAAAGAAGACATTCAAAAAG

CACAAGTGTCTGGACAAGGCGATAGTTTACATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAA

AAAAGGTATTTTACAGACTGTAAAAGTTGTTGATGAATTGGTCAAAGTAATGGGGCGGCATAAGCCAGAA

AATATCGTTATTGAAATGGCACGTGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGTA

TGAAACGAATCGAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCTGTTGAAAATAC

TCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCAAAATGGAAGAGACATGTATGTGGACCAAGAA

TTAGATATTAATCGTTTAAGTGATTATGATGTCGATCACATTGTTCCACAAAGTTTCCTTAAAGACGATT

CAATAGACAATAAGGTCTTAACGCGTTCTGATAAAAATCGTGGTAAATCGGATAACGTTCCAAGTGAAGA

AGTAGTCAAAAAGATGAAAAACTATTGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTT

GATAATTTAACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAAT

TGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAATTTTGGATAGTCGCATGAATACTAAATACGA

TGAAAATGATAAACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCTAAATTAGTTTCTGACTTCCGA

AAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAATTACCATCATGCCCATGATGCGTATCTAAATG

CCGTCGTTGGAACTGCTTTGATTAAGAAATATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTATAA

AGTTTATGATGTTCGTAAAATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAATATTTC

TTTTACTCTAATATCATGAACTTCTTCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGCC

CTCTAATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGATTTTGCCACAGTGCG

CAAAGTATTGTCCATGCCCCAAGTCAATATTGTCAAGAAAACAGAAGTACAGACAGGCGGATTCTCCAAG

GAGTCAATTTTACCAAAAAGAAATTCGGACAAGCTTATTGCTCGTAAAAAAGACTGGGATCCAAAAAAAT

ATGGTGGTTTTGATAGTCCAACGGTAGCTTATTCAGTCCTAGTGGTTGCTAAGGTGGAAAAAGGGAAATC

GAAGAAGTTAAAATCCGTTAAAGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAAT

CCGATTGACTTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACTTAATCATTAAACTACCTAAAT

ATAGTCTTTTTGAGTTAGAAAACGGTCGTAAACGGATGCTGGCTAGTGCCGGAGAATTACAAAAAGGAAA

TGAGCTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGTCATTATGAAAAGTTGAAGGGT

AGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAGCAGCATAAGCATTATTTAGATGAGATTATTG

AGCAAATCAGTGAATTTTCTAAGCGTGTTATTTTAGCAGATGCCAATTTAGATAAAGTTCTTAGTGCATA

TAACAAACATAGAGACAAACCAATACGTGAACAAGCAGAAAATATTATTCATTTATTTACGTTGACGAAT

CTTGGAGCTCCCGCTGCTTTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAAG

AAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATTGATTTGAGTCA

GCTAGGAGGTGACtaactcgagtaaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagact

gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg

tggGCCTTTCTGCGTTTATACCTAGGGAGACGTCGGCGTTCGGCTGCGGCGAGCGGTATcagctcactca

aaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagc

aaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagca

tcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc

cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcc

cttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctc

caagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt

gagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcga

ggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatt

tggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa

accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaag

aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt

catgactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccag

atggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacag

ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctga

ctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgc

gagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaag

tggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcg

ccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggta

tggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc

ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatg

gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaa

ccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataatac

cgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaagg

atcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttta

ctttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgac

acggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctc

atgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttcCGCGCACATTTCCCCGAA

For your References section:
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860

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