出品公司: | Clontech |
---|---|
载体名称: | pLVX-TRE3G , pLVX TRE3G |
质粒类型: | 哺乳动物细胞慢病毒表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | TRE3GV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7819 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | 嘌呤霉素(Puromycin) |
备注: | 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体。 |
产品目录号: | 631191 |
稳定性: | / |
组成型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
pLVX-TRE3G慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorLVX-TRE3G
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-TRE3G 7819 bp DNA circular 31-MAY-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No.631191, 631193,631187, 631189 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers misc_feature 1..635 /label=5'\LTR misc_feature 685..822 /label=Packaging\signal misc_feature 1303..1536 /label=RRE misc_feature 2028..2151 /label=cPPT/CTS misc_feature 2600..3103 /label=pPGK misc_feature 3124..3723 /label=Puro(R) misc_feature 3741..4332 /label=WPRE 3'clip 4536..5172 /label=3'\LTR misc_feature complement(5642..6312) /label=pUC\ori misc_feature 2556..2599 /label=MCS misc_feature 2210..2555 /label=pTRE3G misc_feature complement(6457..7317) /label=Amp(R) BASE COUNT 1973 a 1955 c 2040 g 1851 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 2521 ctggagcaat tccacaacac ttttgtctta tacttggatc cgggcccgcg gccgccccgg 2581 gacgcgtcat atggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 2641 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 2701 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 2761 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 2821 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 2881 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 2941 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 3001 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 3061 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt 3121 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 3181 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 3241 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 3301 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 3361 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 3421 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 3481 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 3541 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 3601 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 3661 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 3721 tgacggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg 3781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 3841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 3901 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 3961 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 4021 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 4081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 4141 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 4201 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 4261 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 4321 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag 4381 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga 4441 ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg 4501 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac 4561 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc 4621 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc 4681 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt 4741 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt 4801 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga 4861 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg 4921 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt 4981 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 5041 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 5101 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 5161 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 5221 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta 5281 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 5341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 5401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 5461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 5521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 5821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 5881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 5941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6121 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 6181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt 6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 6421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 6481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 6541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 6601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 6661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 6721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 6781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 6841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 6901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 6961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 7081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 7141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 7201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 7261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 7321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 7381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 7441 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca 7501 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 7561 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag 7621 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag 7681 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 7741 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat 7801 gtactacaaa cttagtagt //
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