pBact-gRNA-Cas9链霉菌基因编辑CRISPR/Cas9载体质粒，含有guide RNA和Cas9元件-BioVector NTCC保藏中心

pBact-gRNA-Cas9

链霉菌基因编辑CRISPR/Cas9载体质粒，含有guide RNA和Cas9元件于一身。

Streptomyces expression of codon-optimized Cas9 and custom gRNA

pKC1139

• Modifications to backbone
  mutation G8694C to remove BbsI site from pSG5 rep
• Vector type
  Bacterial Expression, CRISPR

• 
  

• Bacterial Resistance(s)
  Apramycin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

图谱：

序列：

gccctgcaggcggaagtcaggtagacacgacttccgctagtccttgcaaggtctgctgacgtgaggcggg

gcggtcgtttttgaccgccctgccttcgtcatgtaggctcgctcgctgtgcctggcgtgtcatcagacgc

ccaggtcccggtgccgtgaggcccgggccatcgagccggtggtacgtggctgcggtccccttgtgagggc

tgcgcgccgtgtgctgtccggcgcgcacagccttgaatccacccgcgggggccggccggtctccgtgagc

tcgagtagacgacggagacgtaatggacaagaagtacagcatcggcctggacatcggcaccaacagcgtg

ggctgggcggtcatcaccgacgagtacaaggtcccctccaagaagttcaaggtcctgggcaacaccgacc

ggcactcgatcaagaagaacctgatcggcgccctgctcttcgacagcggcgaaaccgccgaggcgacccg

cctgaagcggaccgcccgtcgccgctacacccggcgcaagaaccgcatctgctacctgcaggagatcttc

tccaacgagatggccaaggtcgacgactcgttcttccaccggctcgaggagagcttcctggtggaggagg

acaagaagcacgagcgccacccgatcttcggcaacatcgtcgacgaggtggcctaccacgagaagtaccc

caccatctaccacctccgcaagaagctggtcgactcgaccgacaaggcggacctgcggctcatctacctg

gccctcgcgcacatgatcaagttccgcggccacttcctcatcgagggcgacctgaacccggacaactccg

acgtcgacaagctcttcatccagctggtgcagacctacaaccagctgttcgaggagaaccccatcaacgc

cagcggcgtcgacgccaaggcgatcctctccgcgcgcctgagcaagtcccggcgcctggagaacctcatc

gcccagctgccgggcgagaagaagaacggcctcttcggcaacctgatcgcgctgtcgctcggcctgaccc

ccaacttcaagagcaacttcgacctggccgaggacgcgaagctccagctgtccaaggacacctacgacga

cgacctggacaacctgctcgcccagatcggcgaccagtacgcggacctcttcctggccgcgaagaacctc

tcggacgccatcctgctcagcgacatcctgcgggtcaacaccgagatcaccaaggccccgctgtcggcga

gcatgatcaagcggtacgacgagcaccaccaggacctgaccctgctcaaggccctcgtgcgccagcagct

gcccgagaagtacaaggagatcttcttcgaccagtccaagaacggctacgccggctacatcgacggcggc

gcgtcgcaggaggagttctacaagttcatcaagccgatcctggagaagatggacggcaccgaggagctgc

tcgtcaagctgaaccgcgaggacctgctccgcaagcagcggaccttcgacaacggctccatcccgcacca

gatccacctgggcgagctccacgccatcctccggcgccaggaggacttctaccccttcctgaaggacaac

cgcgagaagatcgagaagatcctgaccttccggatcccgtactacgtcggccccctggcccgcggcaact

cccggttcgcgtggatgacccggaagtcggaggaaaccatcaccccgtggaacttcgaggaggtcgtgga

caagggcgcctccgcgcagtcgttcatcgagcgcatgaccaacttcgacaagaacctcccgaacgagaag

gtcctgcccaagcacagcctgctctacgagtacttcaccgtgtacaacgagctgaccaaggtcaagtacg

tgaccgagggcatgcggaagccggccttcctgtccggcgagcagaagaaggcgatcgtcgacctgctctt

caagaccaaccgcaaggtcaccgtgaagcagctgaaggaggactacttcaagaagatcgagtgcttcgac

tccgtcgagatctcgggcgtggaggaccgcttcaacgcctccctgggcacctaccacgacctgctcaaga

tcatcaaggacaaggacttcctcgacaacgaggagaacgaggacatcctggaggacatcgtcctcaccct

gaccctcttcgaggaccgcgagatgatcgaggagcggctcaagacctacgcccacctgttcgacgacaag

gtgatgaagcagctgaagcggcgccggtacaccggctggggccgcctctcccggaagctgatcaacggca

tccgggacaagcagagcggcaagaccatcctggacttcctcaagtccgacggcttcgccaaccgcaactt

catgcagctcatccacgacgactcgctgaccttcaaggaggacatccagaaggcccaggtgtccggccag

ggcgacagcctccacgagcacatcgccaacctggcgggctccccggcgatcaagaagggcatcctccaga

ccgtcaaggtcgtggacgagctggtcaaggtgatgggccgccacaagcccgagaacatcgtgatcgagat

ggcccgggagaaccagaccacccagaagggccagaagaactcccgcgagcggatgaagcgcatcgaggag

ggcatcaaggagctcggctcgcagatcctgaaggagcacccggtcgagaacacccagctccagaacgaga

agctgtacctctactacctgcagaacggccgcgacatgtacgtggaccaggagctcgacatcaaccggct

gagcgactacgacgtcgaccacatcgtgccgcagtccttcctgaaggacgactcgatcgacaacaaggtc

ctgacccgctccgacaagaaccggggcaagtccgacaacgtgccctcggaggaggtcgtgaagaagatga

agaactactggcgccagctgctcaacgccaagctcatcacccagcgcaagttcgacaacctgaccaaggc

cgagcggggcggcctgtcggagctcgacaaggcgggcttcatcaagcgccagctcgtcgaaacccggcag

atcaccaagcacgtggcccagatcctggacagccggatgaacaccaagtacgacgagaacgacaagctga

tccgcgaggtcaaggtgatcaccctcaagagcaagctggtgtccgacttccgcaaggacttccagttcta

caaggtccgggagatcaacaactaccaccacgcccacgacgcgtacctgaacgccgtcgtgggcaccgcg

ctgatcaagaagtacccgaagctggagtccgagttcgtctacggcgactacaaggtctacgacgtgcgca

agatgatcgccaagtcggagcaggagatcggcaaggccaccgcgaagtacttcttctacagcaacatcat

gaacttcttcaagaccgagatcaccctggccaacggcgagatccgcaagcggcccctgatcgaaaccaac

ggcgaaaccggcgagatcgtctgggacaagggccgcgacttcgccaccgtccggaaggtgctgtccatgc

cgcaggtcaacatcgtcaagaaaaccgaggtgcagaccggcggcttcagcaaggagtccatcctccccaa

gcgcaactcggacaagctgatcgcccggaagaaggactgggacccgaagaagtacggcggcttcgacagc

cccaccgtcgcctactccgtgctggtcgtggcgaaggtcgagaagggcaagagcaagaagctgaagtccg

tgaaggagctgctcggcatcaccatcatggagcgctcctcgttcgagaagaacccgatcgacttcctgga

ggccaagggctacaaggaggtcaagaaggacctcatcatcaagctgcccaagtactcgctgttcgagctc

gagaacggccgcaagcggatgctcgccagcgcgggcgagctgcagaagggcaacgagctggccctcccgt

ccaagtacgtcaacttcctgtacctcgcgtcccactacgagaagctgaagggctcgcccgaggacaacga

gcagaagcagctcttcgtggagcagcacaagcactacctggacgagatcatcgagcagatctcggagttc

agcaagcgggtcatcctggccgacgcgaacctcgacaaggtgctgtccgcctacaacaagcaccgcgaca

agccgatccgggagcaggcggagaacatcatccacctgttcaccctcaccaacctgggcgcccccgccgc

gttcaagtacttcgacaccaccatcgaccgcaagcggtacaccagcaccaaggaggtcctcgacgcgacc

ctgatccaccagtccatcaccggcctgtacgaaacccgcatcgacctctcccagctcggcggcgactgag

aattcagatctacgcgttccccgcaaaagcggcctttgactccctgcaagcctcagcgaccgaatatatc

ggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattca

cctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttgctgctccttcg

gtcggacgtgcgtctacgggcaccttaccgcagccgtcggctgtgcgacacggacggatcgggcgaactg

gccgatgctgggagaagcgcgctgctgtacggcgcgcaccgggtgcggagcccctcggcgagcggtgtga

aacttctgtgaatggcctgttcggttgctttttttatacggctgccagataaggcttgcagcatctgggc

ggctaccgctatgatcggggcgttcctgcaattcttagtgcgagtatctgaaaggggatacgcaagtctt

ctcagccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcg

ggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgcca

gggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcg

aattgggtaccgggccccccctcgaggtcctccagcttttgttccctttagtgagggttaattgcgcgct

tggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacg

agccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgc

tcactgcccgctttccaccggtgaagacatgttttagagctagaaatagcaagttaaaataaggctagtc

cgttatcaacttgaaaaagtggcaccgagtcggtgctttttactccatctggatttgttcagaacgctcg

gttgccgccgggcgttttttatctagaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccat

aggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggac

tataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttac

cggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctc

agttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg

ccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac

tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac

ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttg

gtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattac

gcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa

aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttggttcatg

tgcagctccatcagcaaaaggggatgataagtttatcaccaccgactatttgcaacagtgccgttgatcg

tgctatgatcgactgatgtcatcagcggtggagtgcaatgtcgtgcaatacgaatggcgaaaagccgagc

tcatcggtcagcttctcaaccttggggttacccccggcggtgtgctgctggtccacagctccttccgtag

cgtccggcccctcgaagatgggccacttggactgatcgaggccctgcgtgctgcgctgggtccgggaggg

acgctcgtcatgccctcgtggtcaggtctggacgacgagccgttcgatcctgccacgtcgcccgttacac

cggaccttggagttgtctctgacacattctggcgcctgccaaatgtaaagcgcagcgcccatccatttgc

ctttgcggcagcggggccacaggcagagcagatcatctctgatccattgcccctgccacctcactcgcct

gcaagcccggtcgcccgtgtccatgaactcgatgggcaggtacttctcctcggcgtgggacacgatgcca

acacgacgctgcatcttgccgagttgatggcaaaggttccctatggggtgccgagacactgcaccattct

tcaggatggcaagttggtacgcgtcgattatctcgagaatgaccactgctgtgagcgctttgccttggcg

gacaggtggctcaaggagaagagccttcagaaggaaggtccagtcggtcatgcctttgctcggttgatcc

gctcccgcgacattgtggcgacagccctgggtcaactgggccgagatccgttgatcttcctgcatccgcc

agaggcgggatgcgaagaatgcgatgccgctcgccagtcgattggctgagctcatgagcggagaacgaga

tgacgttggaggggcaaggtcgcgctgattgctggggcaacacgtggagcggatcggggattgtctttct

tcagctcgctgatgatatgctgacgctcaatgccgtttggcctccgactaacgaaaatcccgcatttgga

cggctgatccgattggcacggcggacggcgaatggcggagcagacgctcgtccgggggcaatgagatatg

aaaaagcctgaactcaccgcgacgtatcgatgtcgaggttcctcaggggagccaccccagagaagccctc

ggagctgagcggagctatttccaaagccatgccagctagagacagtgcacaccgccaagcatctgcaaaa

ccctcgcctggagggaaagtgcaatgtacgtacgctggtttccctccagaaagggattctgcggcttctc

aactttggaagaagaggcggaacgaactgctgtttcagcctactcatgtgaggaggctggtcctttaccc

tgatgcccgagggcatcagggtaaaggaccagcctcgccaactaggagcgctaccaaggcgaacacaacc

caggacttggtagaacccttcgccggagaagttcagactcatcggcatgagcacccctcgccgcgcactc

ggcaccggtccgtcgaccaccaccaggacgtcgttgtcgacgtcggccccgcggctcctgcccgccgaac

gcgtcgtcgtcgacggcctggtgctcatcgacgagcacccggagccaggtgaaaagcgccggcggacgct

cggactgggcgcgggattccagcagtaacccaggtccgccggccacctcacggcaggcagaccctcggct

ttcgcgccgggaccgccatgagaccgccacccggatgtccggggtggcggtctcatggcggtccctcagc

ggccctacgcgaccgctgtgtcgacgcggaggcagtctccggggagccggccccagggctggagtaccgg

ggcgagcttgcccttgcagcggcggcacacgggcgacgcggcgccgggcgggggagtcttgacgtcgacg

gtgaccggcttcggcttcaggcgcttccgaaggctgatcgtcgggcggatcgcctccttcttcggctgac

gcactcggtcgagcgaagcggccagctcctccgtgcgggcctcgttggccttccggcgcgcctcgcggaa

agcagcttcctcctcggacatgacctcgaacctcatctggtcagcgtcaaggtcgcccggcgcggccggc

gcttccggggcgggcgggtccaggacgtccttgccacacaccaggccccaggactcgacgagccgccgga

cgcccggtaggccgtacgtctcggcgaccttgatgaggtcgaggcgacgtccggcgacgcgggcgatgta

tcggtaccagatgtaggccgggatgaccgcgatggcgaccaggccctcggtgtcgtcggtgatctcctcc

tcggtgcggacgtcctgctggatgccgagttccttgatcagccggttcaggttctgcgaccggtagtgct

tgcggacctggaacacgccgaactcgcgctcgcggtacttctcgacgaacgggccgggccgacgaagccg

ctgcagctcggcggccgccgcgtcgcccaggtcgagcggtcccatgcggtcgtcgccgcgaccggccttg

aagttctgtccggccagctccaggccgatcttggagacgccgcccttggtcttgtcgccgtccttgtaga

ggtagcgggcctgcttgcccgcatcgccgtcagcggcgtccgcgccgttgagtgggcgcacgtcggtgcc

gtggcccttgccctcacaggagcaaccgggccggtcgcacgtcttgctgacggtgtagccgcccgcggat

tcgaccccggcggcccaggctccggcgagtgcgtcgtggaacgcggcctgggcgtccgggccgagcacct

cgcgggtgacccagagcgtgtgccagtgcaggtgccagccggagccccagccgaaggtgtcctcgaaggc

ccgctcgtagccgatgatcccgaagtcgtcgcgcatcgtgcgccagcggcggccggacgagccgtacgcg

cccttccagccgtcgtgcaagaccgcgaccaggccgtgccgcattcccttgcggacggtgccgaacgcca

tgcgctcgaagtggcgcaacgtgttcgtgccaaggtgcagcccgtacccggcgtccgcgagaccgtcggc

ggcgagctgcacgttcgagccccgtacggccaggatgcggctcatgcaccacgggcaggtgtggacgttg

ttgcagcggcacgtgttgccccacgtcgcctcgcccggcttccacatcagctcggccgtcccggcagtga

gccgggtcccgcagcccttgaacgcctcgttcagcgacaccgtctggtgccggtcccgccgggcgaaccg

ctcgtcgcgcgggtcctcccgcccggctgtcgcggcaccctcgtttggggtagaacccgttccagttaca

gcgctctgacctgcagtggacggagattttcccttactactaaagcccgcgtccggattacccgctgtag

tcgtgcttgctacgctgcgtgactggtccgcaatgagacgctttgcgcgctttcggcaggcgtccgagca

gtagattttggggcgcttcccggggatgtggacgatcggggtgccgcagtggcacttcggtccggcgggg

cgcggtggtgtcgacgcgctgttctctcgtacgctcgtcacagagcaaacgtcctcactcggcatgctgc

gccagttgcgggggcggcgagcccgggaggccaatcccgggctcgtgccatttctgggtcctgttgatcc

tggcattggtgtggccgttcattgcccctgctcgctcctgacgcgccgatagacgtccgatacgcccggt

gctggtgggatttgataggtcggaagaagccccgcccgggcctgggcggggcttcctgtgcgtcaggacc

tcctcgtcgtgagcctcttcggcctatggacggagtgacctcgtgatccgttacagccgcgcgcgctcgc

gtagagcggtctcatcagttccacgaacggtcctcttcgcagatcagggcgttggggcggagtctcacca

aggactacgtctgctggcgatttccgttacaccccgggcggtggccggcgcacacgcgcgcccgcgttgg

gcagtgcagaaagtgcagaaacctaggcgctgatggtccaggtccacggttcgtcgtcggcggcggcgcg

ggcggcggcgtcggccagggcgcgggcgagaccggctacggcgggcttgatgcgccggttgcgggcgacc

ttgagcagctagtatgcaggtcgacggatcttttccgctgcataaccctgcttcggggtcattatagcga

ttttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttcc

ttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttc

ttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccggctaccg

ccggcgtaacagatgagggcaagcggatggctgatgaaaccaagccaaccaggaagggcagcccacctat

caaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctg

tcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcg

agctg

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