pLenti6.3-MCS-IRES2-EGFP， pLenti6.3-MCS-IRES2-DsRed2

Biovector Science Lab可提供近万种质粒载体、菌株、基因和细胞株，做为美国质粒载体基因菌株库存中心驻分库及唯一办事处，Biovector Science Lab可为您提供便捷一站式的质粒载体菌株产品服务，订购，报关，缴税，运输，复杂文件申报签署全程跟踪。Biovector并可提供实验技术外包服务，如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi、蛋白质组学研究、色谱/质谱鉴定等服务。您一定能从这找到所需要的资源和技术服务。如有需要请联系：
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质粒载体菌株细胞株基因保藏中心-Biovector Science Lab,Inc.可提供数万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。如有需要请联系： Dr Xu,Senior Scientist 电邮Biovector@163.com 电话1890I268599 订购合同下载请点击：Biovector质粒载体菌株细胞基因订购合同-Biovector Science Lab ^_^寻找您所需质粒载体菌株基因细胞株等资源的窍门：在Google，Baidu等搜索引擎中搜索“Biovector 您所需的资源名称”结果即刻闪现，马上试试吧^_^ 点击浏览器菜单“编辑”-->"查找"或者直接按“Ctrl+F”查找您所需资源 由于页面字数限制，尚有很多质粒载体菌株基因片段名称未列出，如查找不到您所需资源，请发邮件或电话查询。 Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com 标签：以下资源由Biovector Inc. 质粒载体菌株细胞株基因保藏中心独家供应并发布，侵权必究。Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com 质粒 载体 索取 报价 价格 优惠 售价 出售 图谱 序列 酶切位点 转化 构建 求购 购买 订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site Fire Lab C. Elegans Kit (Kit #1000000001) PI: Andrew Fire TALE Toolbox (Kit #1000000019) PI: Feng Zhang Zinc Finger Consortium Expression Vector Kit v1.0 (Kit #1000000010) PI: Keith Joung S. cerevisiae Advanced Gateway Destination Vectors (Kit #1000000011) PI: Susan Lindquist Zinc Finger Consortium Expression Vector and Modular Assembly Kit (Kit #1000000005) PI: Keith Joung Joung Lab REAL Assembly TALEN Kit (Kit #1000000017) PI: Keith Joung Golden Gate TALEN and TAL effector kit 2.0 (Kit #1000000024) PI: Daniel Voytas PPRE X3-TK-luc (1015) Insert: PPRE (Mouse) Tag: NoneMutation: None Expression: MammalianUse: Luciferase Bruce Spiegelman GFP-PGC1 (4) Insert: PGC-1 alpha (Ppargc1a, Mouse) Tag: EGFPMutation: None Expression: MammalianUse: Bruce Spiegelman L4440 (1654) Empty Backbone Tag: T7p, T7p, lacZN, OriF1>>, Ori...Mutation: None Expression: WormUse: RNAi Andrew Fire pBABE-zeo largeTcDNA (1779) Insert: large T antigen (SV40gp6, simian virus) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Bob Weinberg pCI neo-hEST2 (1781) Insert: hTERT (TERT, Human) Tag: NoneMutation: None Expression: MammalianUse: Bob Weinberg pBMN-I-GFP (1736) This retroviral vector has an IRES-GFP downstream of the cloning sites Insert: IRES-GFP () Tag: NoneMutation: None Expression: Use: Retroviral Garry Nolan pBABE-neo (1767) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Retroviral Bob Weinberg pBABE-neo-hTERT (1774) Insert: hTERT (TERT, Human) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Bob Weinberg mTOR_2 shRNA (1856) Insert: mTOR (MTOR, Human) Tag: NoneMutation: None Expression: Use: Lentiviral, RNAi David Sabatini pBABE-puro HGF (10901) Insert: HGF (HGF, Human) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Bob Weinberg pLKO.1 - TRC control (10879) Insert: non-hairpin 18bp () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi David Root GFP-p53 (12091) Insert: p53 (TP53, Human) Tag: GFPMutation: None Expression: MammalianUse: Tyler Jacks pSicoR PGK puro (12084) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Tyler Jacks pCAG-FlpeERT2 (14756) Insert: Flpe-ERT2 fusion protein (Budding Yeast) Tag: ERT2Mutation: None Expression: MammalianUse: Flp/FRT Connie Cepko pSicoR human Dicer1 (14763) Insert: Dicer shRNA (DICER1, Human) Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Tyler Jacks pWZL-Neo-Myr-Flag-DEST (15300) Empty Backbone Tag: Myr, FlagMutation: None Expression: MammalianUse: Retroviral Jean Zhao pLKO.1 puro (8453) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi Bob Weinberg pIRESneo-FLAG/HA Ago4 (10824) Insert: Argonaute 4 (EIF2C4, Human) Tag: FLAG, HAMutation: None Expression: MammalianUse: Thomas Tuschl 1436 pcDNA3 Flag HA (10792) pcDNA3 vector with added Flag, HA tags Empty Backbone Tag: FLAG, HAMutation: None Expression: MammalianUse: William Sellers EGFR L858R (11012) Retroviral construct for expressing human EGFR with L858R mutation in human cells Insert: EGFR L858R (EGFR, Human) Tag: NoneMutation: L858R Expression: MammalianUse: Retroviral Matthew Meyerson pDEST-periHisMBP (11086) Empty Backbone Tag: NoneMutation: None Expression: BacterialUse: David Waugh WT NFAT2 (11101) Insert: NFAT2 (Nfatc1, Mouse) Tag: 3xHA, GFPMutation: None Expression: MammalianUse: Retroviral Anjana Rao pCAG-DsRed (11151) Mammalian expression vector for expression of DsRed Insert: DsRed2 from Discosoma sp () Tag: NoneMutation: None Expression: MammalianUse: Connie Cepko GFP-VIVIT (11106) Insert: VIVIT () Tag: GFPMutation: None Expression: MammalianUse: Anjana Rao M51 Super 8x FOPFlash (TOPFlash mutant) (12457) Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter. Insert: mutant TCF/LEF binding sites (Ctnnb1, ) Tag: NoneMutation: mutant TCF/LEF binding sites Expression: MammalianUse: Luciferase Randall Moon pMXs-Stat3-C (13373) Insert: signal transducer and activator of transcription 3 (dominant active mutant) (Stat3, Mouse) Tag: NoneMutation: Changed Alanine 661 to cystei... Expression: MammalianUse: Retroviral Shinya Yamanaka pMXs-Nanog (13354) Insert: Nanog homeobox (Nanog, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Shinya Yamanaka pMXs-Oct3/4 (13366) Insert: POU domain, class 5, transcription factor 1 (Pou5f1, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Shinya Yamanaka pSicoR (11579) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Tyler Jacks pSico PGK puro (11586) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Tyler Jacks pPGK-Cre-bpA (11543) Insert: Phosphoglycerate kinase 1 promoter (bacteriophage P1) Tag: NoneMutation: None Expression: MammalianUse: Cre/Lox Klaus Rajewsky NFAT/AP-1 3x luciferase (11783) Insert: NFAT/AP-1 3x (Il2, Mouse) Tag: luciferaseMutation: None Expression: BacterialUse: Luciferase Anjana Rao pLL3.7 (11795) 2nd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Luk Parijs pPAGFP-N1 (11909) Insert: pPAGFP-N1 (Aequorea victoria) Tag: NoneMutation: These plasmids are essentiall... Expression: MammalianUse: Jennifer Lippincott-Schwartz pBS185 CMV-Cre (11916) Insert: cre (cre, bacteriophage P1) Tag: NoneMutation: None Expression: MammalianUse: Cre/Lox Brian Sauer pBS598 EF1alpha-EGFPcre (11923) Insert: EGFP-cre (cre, bacteriophage P1) Tag: NoneMutation: None Expression: MammalianUse: Cre/Lox Brian Sauer GFP-Ub (11928) Insert: Ubiquitin (UBC, Human) Tag: GFPMutation: None Expression: MammalianUse: Nico Dantuma mRFP-Ub (11935) Insert: Ubiquitin (Human) Tag: mRFPMutation: None Expression: MammalianUse: Nico Dantuma DsRed-Rab5 DN (13051) Insert: RAB5A (Canis familiaris) (RAB5A, Canis familiaris) Tag: DsRed2, HAMutation: asparagine 3 changed to serin... Expression: MammalianUse: Richard Pagano pcDNA3-mRFP (13032) Insert: Monomer Red Fluorescent Protein () Tag: NoneMutation: None Expression: MammalianUse: Doug Golenbock pcDNA3-TLR4-YFP (13018) Insert: Toll-Like Receptor 4 (TLR4, Human) Tag: YFPMutation: None Expression: MammalianUse: Doug Golenbock pCyPet-His (14030) Insert: CyPet () Tag: 6xHisMutation: None Expression: BacterialUse: Patrick Daugherty FUGW (14883) 3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion Insert: flap-Ub promoter-GFP-WRE () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral David Baltimore pCI flag PCAF (8941) Insert: PCAF (KAT2B, Human) Tag: flagMutation: None Expression: MammalianUse: Yoshihiro Nakatani pRK7-HA-S6K1-WT (8984) Insert: S6K1 (Rps6kb1, Rat) Tag: HAMutation: None Expression: MammalianUse: John Blenis pH3U3-mcs (12609) Insert: Multiple cloning site in His3 Ura3 reporter plasmid (Budding Yeast) Tag: NoneMutation: None Expression: BacterialUse: Scot Wolfe pBabe puro HA PIK3CA (12522) Insert: PIK3CA (PIK3CA, Human) Tag: HAMutation: None Expression: MammalianUse: Retroviral Jean Zhao SIRT5 Flag (13816) Insert: SIRT5 (SIRT5, Human) Tag: flagMutation: None Expression: MammalianUse: Eric Verdin HDAC6 Flag (13823) Insert: HDAC6 (HDAC6, Human) Tag: flagMutation: None Expression: MammalianUse: Eric Verdin GST-RBD (15247) Insert: Rhotekin (Rtkn, Mouse) Tag: GSTMutation: amino acids 7-89 Expression: BacterialUse: Martin Schwartz 3336 pcDNA3 Bcl-2 (8768) Insert: Bcl-2 (BCL2, Human) Tag: NoneMutation: None Expression: MammalianUse: Stanley Korsmeyer pLOX-gfp-iresTK (12243) Insert: GFP, HSV1-TK () Tag: NoneMutation: LoxP in 3 LTR. Expression: MammalianUse: Lentiviral, Cre/Lox Didier Trono pLV-tTRKRAB-red (12250) Insert: TetR-KRAB fusion and dsRED () Tag: NoneMutation: dsRed added to backbone. Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Didier Trono pIRESneo-EGFP-alpha Tubulin (12298) Insert: EGFP-alpha Tubulin (TUBA1B, Human) Tag: EGFPMutation: None Expression: MammalianUse: Patricia Wadsworth eGFP STAT1 WT (12301) Insert: STAT1 (STAT1, Human) Tag: eGFPMutation: None Expression: MammalianUse: Alan Perantoni pGEXTK-Pak1 70-117 (12217) Insert: Pak1 (PAK1, Human) Tag: GSTMutation: PBD (p21-binding domain), aa ... Expression: BacterialUse: Jonathan Chernoff pCMV5-Smad7-HA (11733) Insert: SMAD7 (SMAD7, Human) Tag: HAMutation: None Expression: MammalianUse: Jeff Wrana pLVET-tTR-KRAB (11644) Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation Insert: hEF-1alpha, GFP, tTR-KRAB, Tet-on () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Patrick Aebischer pCMV5B-Flag-Smurf1 wt (11752) Insert: SMURF1 (SMURF1, Human) Tag: FlagMutation: None Expression: MammalianUse: Jeff Wrana pLVUT-tTR-KRAB (11651) Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation Insert: hUbiquitin C, GFP, tTR-KRAB, Tet-on () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Patrick Aebischer GFP-rab11 DN (12678) Insert: RAB11A (Homo sapiens) (RAB11A, Human) Tag: GFP, HAMutation: changed serine 25 to asparagi... Expression: MammalianUse: Richard Pagano DsRed-rab11 DN (12680) Insert: RAB11A (Homo sapiens) (RAB11A, Human) Tag: DsRed, HAMutation: changed serine 25 to asparagi... Expression: MammalianUse: Richard Pagano pcDNA3-EGFP-Cdc42-T17N (12976) Insert: Cdc42 DN (CDC42, Human) Tag: EGFPMutation: T17N Dominant negative Expression: MammalianUse: Gary Bokoch DsRed-rab7 WT (12661) Insert: RAB7 (Homo sapiens) (RAB7A, Human) Tag: DsRed, HAMutation: None Expression: MammalianUse: Richard Pagano pCAG-ERT2CreERT2 (13777) Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen) Insert: ERT2-Cre-ERT2 fusion protein (Bacteriophage P1) Tag: NoneMutation: None Expression: MammalianUse: Cre/Lox Connie Cepko pCMV5 mouse src K295R Y527F (13657) Insert: src K295R Y527F (Src, Mouse) Tag: NoneMutation: K295R Y527F. Dominant negativ... Expression: MammalianUse: Joan Brugge pcDNA3 Flag TSC2 (14129) Insert: TSC2 (TSC2, Human) Tag: FlagMutation: None Expression: MammalianUse: Brendan Manning pcDNA flag PPAR gamma (8895) Insert: PPAR gamma (Pparg, Mouse) Tag: flagMutation: None Expression: MammalianUse: Bruce Spiegelman 1477 pcDNA3 flag HA Akt1 (9021) Insert: Akt1 (AKT1, Human) Tag: flag, HAMutation: None Expression: MammalianUse: William Sellers pBABE puro K-Ras V12 (9052) Retroviral expression of a constitutively active form of K-Ras Insert: KRAS (KRAS, Human) Tag: NoneMutation: G12V. Constitutively active. Expression: MammalianUse: Retroviral William Hahn pMKO.1 puro p53 shRNA 2 (10672) Insert: p53 shRNA (TP53, Human) Tag: NoneMutation: None Expression: MammalianUse: Retroviral, RNAi William Hahn pCDNA3-Flag MKK6(glu) (13518) Insert: MKK6(glu) (MAP2K6, Human) Tag: FlagMutation: Ser-207Glu Thr-211Glu Expression: MammalianUse: Roger Davis KJBAC1 strain (13424) Bacterial Strain Tag: NoneMutation: None Expression: Use: Keith Joung Cav1-mRFP (14434) Insert: Caveolin-1 (CAV1, Canis familalis) Tag: mRFPMutation: None Expression: MammalianUse: Ari Helenius pLenti CMV/TO Puro DEST (670-1) (17293) 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Eric Campeau pMXs-hOCT3/4 (17217) Insert: OCT3/4 (POU5F1, Human) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Shinya Yamanaka pLenti6/UbC/mSlc7a1 (17224) Insert: Slc7a1 (Slc7a1, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Shinya Yamanaka MSCV h c-MYC IRES GFP (18119) Insert: c-MYC (MYC, Human) Tag: GFPMutation: The CUG which is the start si... Expression: Use: Retroviral John Cleveland pLJM1-EGFP (19319) 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral David Sabatini FUW-M2rtTA (20342) Lentiviral plasmid expressing the reverse tetracycline transactivator Insert: M2rtTA (bacteria) Tag: NoneMutation: None Expression: Use: Lentiviral Rudolf Jaenisch FUW-OSKM (20328) Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation Insert: Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Mouse) Tag: NoneMutation: None Expression: Use: Lentiviral Rudolf Jaenisch CMV-Brainbow-1.1 M (18722) Insert: Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean () Tag: NoneMutation: None Expression: MammalianUse: Cre/Lox Joshua Sanes MSCV IRES Luciferase (18760) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Retroviral, Luciferase Scott Lowe pWZL Blast mouse E-cadherin (18804) Insert: E-cadherin (Cdh1, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Bob Weinberg AAV-FLEX-rev-ChR2-tdtomato (18917) Insert: Channelrhodopsin 2-tdtomato (Chlamydomonas reinhardtii) Tag: tdtomatoMutation: None Expression: Use: Cre/Lox Scott Sternson HA-HIF1alpha P402A/P564A-pcDNA3 (18955) Insert: Hypoxia inducible factor 1 alpha (HIF1A, Human) Tag: HA-tagMutation: cDNA changed amino acids: P... Expression: MammalianUse: William Kaelin HA-HIF2alpha-pcDNA3 (18950) Insert: Hypoxia inducible factor 2 alpha (EPAS1, Human) Tag: HA-tagMutation: wt cDNA Expression: MammalianUse: William Kaelin pLenti CMV GFP DEST (736-1) (19732) 3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral, RNAi Eric Campeau FU-tet-o-hc-myc (19775) Insert: c-myc (MYC, Human) Tag: NoneMutation: T58A Expression: MammalianUse: Lentiviral Konrad Hochedlinger pLOVE-Nmyc (15951) Insert: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn,Mouse) Tag: V5Mutation: The recombination reaction re... Expression: Use: Lentiviral Miguel Ramalho-Santos pMXs-Klf4-IP (15920) Insert: Kruppel-like factor 4 (gut) (Klf4, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Shinya Yamanaka pLOVE-GFP (15949) Insert: Green Fluorescent Protein (Aequorea victoria) Tag: V5Mutation: The recombination reaction re... Expression: Use: Lentiviral Miguel Ramalho-Santos pGL3-NFAT luciferase (17870) Insert: 3x NFAT binding sequence () Tag: IL2 promoter, LuciferaseMutation: None Expression: MammalianUse: Luciferase Jerry Crabtree 101-200 of 712 results pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr (19326) Insert: Pmyo-3::twk-18(cn110)cDNA (Nematode) Tag: NoneMutation: None Expression: WormUse: Erik Jorgensen pJL44 - Phsp16.48::MosTase::glh-2utr (19333) Insert: Phsp-16::Mos1 transposase (Nematode) Tag: NoneMutation: None Expression: WormUse: Erik Jorgensen GFP-C1-PLCdelta-PH (21179) encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids Insert: PLCdelta (PLCD1, Human) Tag: GFPMutation: PH domain of human PLC delta.... Expression: MammalianUse: Tobias Meyer pEGFP-LC3 (21073) Insert: microtubule-associated protein 1 light chain 3 beta (Map1lc3b, Rat) Tag: EGFPMutation: First ATG is not included. Expression: MammalianUse: Tamotsu Yoshimori pcDNA/Myc TCF4 (16512) Insert: TCF4 (TCF7L2, Human) Tag: MycMutation: None Expression: MammalianUse: Bert Vogelstein pSin-EF2-Oct4-Pur (16579) Insert: Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1, Human) Tag: NoneMutation: None Expression: MammalianUse: Lentiviral James Thomson pMXs-Oct3/4-IP (15918) Insert: POU domain, class 5, transcription factor 1 (Pou5f1, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Retroviral Shinya Yamanaka Flag p21 WT (16240) Insert: p21 (CDKN1A, Human) Tag: FlagMutation: None Expression: MammalianUse: Mien-Chie Hung pcDNA3 MDM2 WT (16233) Insert: MDM2 (MDM2, Human) Tag: NoneMutation: None Expression: MammalianUse: Mien-Chie Hung c-myc promoter (Del 1) (16601) Insert: c-myc promoter (Del 1) (MYC, Human) Tag: luciferaseMutation: Del-1 (XmnI->PvuII) Expression: MammalianUse: Luciferase Bert Vogelstein ISceI-GR-RFP (17654) Insert: I-SceI-GRLBD (SCEI, Nr3c1, Rat) Tag: mRFPMutation: C655G mutation in the glucoco... Expression: MammalianUse: Tom Misteli EF.hICN1.CMV.GFP (17623) Insert: ICN1 (NOTCH1, Human) Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Linzhao Cheng pRK5-HA-Ubiquitin-K48R (17604) Mammalian expression of HA tagged ubiquitin K48R Insert: Ubiquitin C (UBC, Human) Tag: HAMutation: K48R Expression: MammalianUse: Ted Dawson pCAG2LMKOSimO (20866) Insert: c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Mouse) Tag: NoneMutation: None Expression: MammalianUse: Keisuke Kaji pEP4 E02S CK2M EN2L (20924) Insert: Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (Human) Tag: NoneMutation: None Expression: Use: Episomal/EBNA James Thomson pET21a-Streptavidin-Dead (20859) Insert: Streptavidin-Dead (E. Coli) Tag: NoneMutation: Triple mutant of wt Streptavi... Expression: BacterialUse: Alice Ting HER2 WT (16257) Insert: HER2 (ERBB2, Human) Tag: NoneMutation: None Expression: MammalianUse: Mien-Chie Hung pBAD/myc-his A Rv0712 (FGE) (16132) Bacterial expression of Mycobacterium tuberculosis FGE Insert: Formylglycine-generating enzyme (Mycobacterium tuberculosis) Tag: NoneMutation: None Expression: BacterialUse: Carolyn Bertozzi PG13-luc (wt p53 binding sites) (16442) Insert: p53-binding site (TP53, ) Tag: luciferaseMutation: None Expression: MammalianUse: Luciferase Bert Vogelstein pCS2-Jmjd3-F (17440) Insert: JMJD3 (Kdm6b, Mouse) Tag: 6xMyc, FlagMutation: None Expression: MammalianUse: Kai Ge pLenti CMV Puro DEST (w118-1) (17452) 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Eric Campeau pBigT (21270) Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence. Empty Backbone Tag: NoneMutation: None Expression: Use: Mouse Targeting Frank Costantini PL-SIN-EOS-S(4+)-EiP (21314) Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral James Ellis PL-SIN-Nanog-EGFP (21321) Insert: Enhanced Green Fluorescence Protein () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral James Ellis GW1CMV-Perceval (21737) Insert: Perceval (fluorescent ATP:ADP sensor) (Unknown) Tag: 5UTRMutation: improved Kozak sequence Expression: MammalianUse: Gary Yellen pBABE-puro mCherry-EGFP-LC3B (22418) Insert: microtubule-associated protein 1 light chain 3 beta (MAP1LC3B, Human) Tag: mCherry, eGFPMutation: None Expression: MammalianUse: Retroviral Jayanta Debnath Hsp90 HA (22487) Insert: Hsp90 HA (Human) Tag: HAMutation: None Expression: MammalianUse: William Sessa pQUAST (24349) Insert: 5xQUAS-Pmin promoter (Fly) Tag: NoneMutation: None Expression: InsectUse: Liqun Luo EF.STAT3C.Ubc.GFP (24983) Lentiviral expression of constitutively active Stat3 Insert: STAT3 (STAT3, Human) Tag: FLAGMutation: A661C, N663C Expression: MammalianUse: Lentiviral Linzhao Cheng pCBASceI (26477) I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site Insert: pCBASceI () Tag: HAMutation: None Expression: MammalianUse: Maria Jasin pcDNA3.3_LIN28A (26819) Insert: 5UTR-LIN28A-3UTR (LIN28A, Human) Tag: NoneMutation: None Expression: MammalianUse: Derrick Rossi Tet-pLKO-puro (21915) Empty Backbone Tag: NoneMutation: None Expression: Use: Lentiviral Dmitri Wiederschain pCMV4 p50 (21965) Insert: NF-kB p50 subunit (NFKB1, Human) Tag: NoneMutation: None Expression: MammalianUse: Warner Greene FLAG-TRAF6-wt (21624) Insert: TNF receptor-associated factor 6 (Traf6, Mouse) Tag: FLAGMutation: None Expression: MammalianUse: John Kyriakis pcDNA3.1-p300 (23252) Insert: p300 (EP300, Human) Tag: NoneMutation: None Expression: MammalianUse: Warner Greene pCFJ601 - Peft-3 Mos1 transposase (34874) Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans Insert: Mos1 transpoase (Nematode) Tag: NoneMutation: None Expression: Use: Worm targeting Erik Jorgensen CMV-B-GECO1 (32448) Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging Insert: B-GECO1.0 () Tag: NoneMutation: GCaMP3 S40P/L60P/D86G/K119I/L... Expression: MammalianUse: Robert Campbell pMJ806 (39312) Insert: Cas9 (Streptococcus pyogenes) Tag: MBP, His6Mutation: None Expression: BacterialUse: Jennifer Doudna pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination (40131) Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: Pawel Pelczar pC-GoldyTALEN (38143) destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter Insert: GoldyTALEN (Unknown) Tag: FokI homodimer, AcV5Mutation: Truncate at N and C terminous Expression: MammalianUse: Daniel Carlson 8xGTIIC-luciferase (34615) YAP/TAZ-responsive synthetic promoter driving luciferase expression Insert: synthetic TEAD luciferase reporter () Tag: NoneMutation: None Expression: Use: Luciferase Stefano Piccolo gRNA_Cloning Vector (41824) An empty gRNA expression vector, used to create a gRNA to a specific sequence. Empty Backbone Tag: NoneMutation: None Expression: MammalianUse: George Church gRNA_AAVS1-T1 (41817) Insert: gRNA_AAVS1-T1 () Tag: NoneMutation: None Expression: MammalianUse: George Church pdCas9-humanized (44246) Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown Insert: dead Cas9 with 3X NLS (Human) Tag: 3x NLSMutation: D10A H840A Expression: MammalianUse: Retroviral Stanley Qi pCPRDEnv (1732) This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab Insert: Gag-Pol and Rev (Feline Immunodeficiency Virus) Tag: NoneMutation: None Expression: MammalianUse: Garry Nolan scramble shRNA (1864) 3rd gen lentiviral negative control vector containing scrambled shRNA Insert: scramble () Tag: NoneMutation: None Expression: Use: Lentiviral, RNAi David Sabatini HA-FOXO3a WT (1787) Insert: FOXO3a (FOXO3, Human) Tag: HA (YDVPDYASL)Mutation: None Expression: MammalianUse: Michael Greenberg 408 pSG5L HA E2F1 (10736) Insert: E2F1 (E2F1, Human) Tag: HAMutation: None Expression: MammalianUse: William Sellers 800 pSG5L HA PTEN wt (10750) Insert: PTEN (PTEN, Human) Tag: HAMutation: None Expression: MammalianUse: William Sellers pIRESneo-FLAG/HA Ago1 (10820) Insert: Argonaute 1 (AGO1, Human) Tag: FLAG, HAMutation: None Expression: MammalianUse: Thomas Tuschl 1245 pCMVb p300 (10717) Insert: p300 (EP300, Human) Tag: NoneMutation: None Expression: MammalianUse: William Sellers pTA-Luc SIRT1 promoter (-202) (10971) Insert: SIRT1 promoter (Sirt1, Mouse) Tag: NoneMutation: None Expression: MammalianUse: Luciferase Toren Finkel pLB (11619) pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added. Empty Backbone Tag: GFPMutation: None Expression: MammalianUse: Lentiviral, RNAi, Cre/Lox Stephan Kissler pLVPT-rtTR-KRAB-2SM2 (11652) Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation Insert: mPGK, GFP, rtTR-KRAB-2SM2, Tet-off () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Patrick Aebischer pET16B.Pfu (12509) Insert: Pfu DNA polymerase (P. furiosus) Tag: NoneMutation: Y218H, Q462R, F533L, K559I an... Expression: BacterialUse: Sung-Hou Kim pBabe puro Myr HA PIK3CA (12523) Insert: PIK3CA Myr (PIK3CA, Human) Tag: Myr, HAMutation: Myristoylation. Constitutivel... Expression: MammalianUse: Retroviral Jean Zhao pcDNA3-Myd88-CFP (13026) Insert: myeloid differentiation primary response gene (88) (MYD88, Human) Tag: CFPMutation: None Expression: MammalianUse: Doug Golenbock pcDNA3-YFP (13033) Insert: Yellow fluorescent protein () Tag: NoneMutation: None Expression: MammalianUse: Doug Golenbock pcDNA3-EGFP-RhoA-wt (12965) Insert: RhoA (RHOA, Human) Tag: EGFPMutation: None Expression: MammalianUse: Gary Bokoch SIRT1 Flag (13812) Insert: SIRT1 (SIRT1, Human) Tag: flagMutation: N-terminus truncated Expression: MammalianUse: Eric Verdin pFLPo (13792) Insert: FLPo () Tag: NoneMutation: None Expression: BacterialUse: Cloning vector Philippe Soriano SIRT6 Flag (13817) Insert: SIRT6 (SIRT6, Human) Tag: flagMutation: None Expression: MammalianUse: Eric Verdin HDAC7 Flag (13824) Insert: HDAC7 (HDAC7, Human) Tag: flagMutation: None Expression: MammalianUse: Eric Verdin p212 pCMV-EGFP/RFP (8389) Insert: floxed EGFP () Tag: NoneMutation: None Expression: MammalianUse: Cre/Lox Jeffrey Green pSG5 Large T (9053) Insert: large T antigen (SV40gp6, simian virus) Tag: NoneMutation: None Expression: MammalianUse: William Hahn pBABE-hygro p53 DD (9058) Insert: p53 DD (Trp53, Mouse) Tag: NoneMutation: DD. Dominant negative mutant... Expression: MammalianUse: Retroviral Bob Weinberg 1036 pcDNA3 Myr HA Akt1 (9008) Mammalian expression of human Akt1 with myr, HA tags Insert: Akt1 (AKT1, Human) Tag: myr, HAMutation: None Expression: MammalianUse: William Sellers 1098 pcDNA GFP FKHR (9022) Insert: FKHR (FOXO1, Human) Tag: GFPMutation: None Expression: MammalianUse: William Sellers STOP-eGFP-ROSA26TV (11739) Insert: STOP-eGFP-ROSA26TV (DNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)) Tag: NoneMutation: None Expression: Use: Cre/Lox Klaus Rajewsky pEGFP-VSVG (11912) Insert: VSVG () Tag: NoneMutation: F64L, S65T, A206K relative th... Expression: MammalianUse: Jennifer Lippincott-Schwartz pMDLg/pRRE (12251) Insert: HIV-1 GAG/POL () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Didier Trono pCMV delta R8.2 (12263) Insert: HIV-1 GAG/POL, Tat and Rev () Tag: NoneMutation: None Expression: MammalianUse: Lentiviral Didier Trono pMIG-w (12282) a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus Empty Backbone Tag: IRES GFPMutation: None Expression: MammalianUse: Retroviral Luk Parijs pCMV-HA-MyD88 (12287) Insert: MyD88 (MYD88, Human) Tag: HAMutation: None Expression: MammalianUse: Bruce Beutler pmCherry-ATG5 (13095) Insert: ATG5 (Atg5, Mouse) Tag: mCherryMutation: None Expression: MammalianUse: Roberta Gottlieb pcDNA3 Flag FKHR (13507) Insert: FKHR (FOXO1, Human) Tag: FlagMutation: None Expression: MammalianUse: Kunliang Guan Cdc2-HA (1888) Insert: Cdc2 (CDK1, Human) Tag: HAMu

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