Renilla luciferase-Pol III (BioVector-037380) BioVector NTCC质粒载体菌种细胞基因保藏中心

Renilla luciferase-Pol III (BioVector-037380) BioVector NTCC质粒载体菌种细胞基因保藏中心 Perrimon Lab plasmid #414 The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.
Vector backbone pRL-null (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 3320 Vector type Mammalian Expression, Luciferase GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name PolIII-Renilla control reporter Alt name RNA polymerase III control promoter Alt name RNA PolIII 128 subunit (RpIII128) Alt name Renilla luciferase Species D. melanogaster (fly) GenBank IDAE003823 Entrez Gene RpIII128 (a.k.a. Dmel_CG8344, CG8344, DmRP128, Dmel\CG8344, RP128) Promoter RNA PolIII 128 subunit (RpIII128) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site BglII (unknown if destroyed) 3′ cloning site SpeI (unknown if destroyed) 5′ sequencing primer T7 3′ sequencing primer EBV-rev
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