NRRL3143 BioVector NTCC质粒载体菌种细胞基因保藏中心

NRRL3143 BioVector NTCC质粒载体菌种细胞基因保藏中心 Streptomyces refuineus subsp. thermotolerans" Taxonomy in NCBI database: Bacteria; Actinobacteria; Actinobacteria; Streptomycetales; Streptomycetaceae. 4933 <-- KCC S-0933 <-- Kyowa Hakko Kogyo Co., Ltd.; KY 2361 <-- NRRL 3143 <-- Hoffmann-La Roche Inc.; 401-9T-22. Accessioned in 1983. =NRRL 3143. Medium: 42; Temperature: 37°C; Rehydration fluid: 656. Production: Anthramycin (refuin, acrylamides) [1443]. Delivery category: Domestic, A or C; Overseas, A or C. Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
Production: Anthramycin (refuin, acrylamides) Medium: YEAST-STARCH AGAR (A) Yeast extract (BD-Difco) 2.0 g Soluble starch 10.0 g Agar 15.0 g Distilled water 1.0 L Adjust pH to 7.3. Temperature: 37°C; Rehydration fluid: TRYPTONE-YEAST EXTRACT BROTH (ISP-1) WITH 0.2% MgSO4·7H2O Tryptone (BD-Difco) 5.0 g Yeast extract (BD-Difco) 3.0 g MgSO4·7H2O 2.0 g Distilled water 1.0 L Adjust pH to 7.0 - 7.2. Comment: The premixed powder of Tryptone-yeast extract broth is available from Becton Dickinson & Co. as ISP Medium 1. Add MgSO4·7H2O to the medium before autoclaving.
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