pDPH5 BioVector NTCC质粒载体菌种细胞基因保藏中心

pDPH5 BioVector NTCC质粒载体菌种细胞基因保藏中心 pDPH5, PDI expression plasmid, trp promoter, colE1 origin, Amp R; pCT54, Control plasmid for pDPH5, trp promoter, colE1 origin, Amp R, The PDI vector pDPH5 contains an OmpA-PDI gene fusion under the control of the trp promoter. This results in the efficient expression of mature PDI to the periplasm of E. coli. when the cells are grown in the presence of the inducer IAA, as described previousl. This plasmid is compatible with those encoding the Fab's, enabling use of strains bearing both plasmids to investigate the effect of PDI on the folding of Fab's in the E. coli periplasm. Plasmid pDPH5 was shown to express PDI to the E. coli periplasm. Cells containing pDPH5 grown in the presence of the inducer IAA expressed a new protein in periplasmic fractions produced by osmotic shock with an apparent molecular mass of 57.4 kDa. Western blot analysis with a polyclonal rabbit anti-bovine PDI antibody showed that PDI was produced in the DsbA strains used for further experimentation BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net