pET-Cas9-NLS-6xHis (BioVector-062933) BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥9825
- 货 号：pET-Cas9-NLS-6xHis (Plasmid #62933)
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pET-Cas9-NLS-6xHis
(BioVector-062933) BioVector NTCC质粒载体菌种细胞基因保藏中心 Expression of Cas9-NLS-6xHis in bacterial cells
Vector backbone pET29 (Search Vector Database) Backbone manufacturer Novagen Backbone size w/o insert (bp) 5161 Total vector size (bp) 9295 Vector type Bacterial Expression GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) Mach1 Growth instructions For protein expression, transform into BL21 Star (DE3) cells. Induce at 0.6 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C. Copy number High Copy GENE/INSERT Gene/Insert name Cas9-NLS-6xHis Species S. pyogenes Insert Size (bp) 4152 Promoter T7 Tags / Fusion Proteins 6xHis tag (C terminal on backbone) NLS (C terminal on insert) CLONING INFORMATION Cloning method Gibson Cloning 5′ sequencing primer T7 promoter 3′ sequencing primer T7 terminator (Common Sequencing Primers) RESOURCE INFORMATION Supplemental Documents pET-Cas9-NLS-6xHis A portion of this plasmid was derived from a plasmid made by pMJ806 served as template for Cas9 gene. This was reported in Pattanayak et. al. Nature Biotechnology 2013.
Alternative plasmid name: pJAZ0005
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net
Vector backbone pET29 (Search Vector Database) Backbone manufacturer Novagen Backbone size w/o insert (bp) 5161 Total vector size (bp) 9295 Vector type Bacterial Expression GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) Mach1 Growth instructions For protein expression, transform into BL21 Star (DE3) cells. Induce at 0.6 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C. Copy number High Copy GENE/INSERT Gene/Insert name Cas9-NLS-6xHis Species S. pyogenes Insert Size (bp) 4152 Promoter T7 Tags / Fusion Proteins 6xHis tag (C terminal on backbone) NLS (C terminal on insert) CLONING INFORMATION Cloning method Gibson Cloning 5′ sequencing primer T7 promoter 3′ sequencing primer T7 terminator (Common Sequencing Primers) RESOURCE INFORMATION Supplemental Documents pET-Cas9-NLS-6xHis A portion of this plasmid was derived from a plasmid made by pMJ806 served as template for Cas9 gene. This was reported in Pattanayak et. al. Nature Biotechnology 2013.
Alternative plasmid name: pJAZ0005
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net
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