Sp2/0-Ag14 (ATCC CRL-1581) BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥29725
- 货 号：Sp2/0-Ag14 (ATCC CRL-1581)
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Sp2/0-Ag14 (ATCC CRL-1581) BioVector NTCC质粒载体菌种细胞基因保藏中心 Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain BALB/c
Applications
This cell line is a fusion partner for production of somatic cell hybrids.
Images Derivation The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. Antigen Expression Antigen expression: H-2d Comments The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 μg/ml and are HAT sensitive. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 104 and 5 X 105 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density) Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C
U.S. Patent Number 4,945,057 References Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net
Images Derivation The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. Antigen Expression Antigen expression: H-2d Comments The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 μg/ml and are HAT sensitive. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 104 and 5 X 105 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density) Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C
U.S. Patent Number 4,945,057 References Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net
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