pzhen18b vector BioVector NTCC质粒载体菌种细胞基因保藏中心

pzhen18b vector BioVector NTCC质粒载体菌种细胞基因保藏中心 Development of a Male Sterility System Using OsNP1. Because the osnp1-1 mutant exhibited traits desirable to the male sterile line, we sought to develop a nuclear male sterility system using the mutant plant and OsNP1 gene. The strategy is shown in Fig. S7A. We transformed osnp1-1 with a double T-DNA binary vector pZhen18B (Fig. S7B). The first T-DNA contained NPTII under the CaMV 35S promoter for transformation selection. The second T-DNA contained three functional modules: OsNP1 under its native promoter for restoration of male fertility, the maize α-amylase gene ZM-AA1 under the pollen-specific PG47 promoter to devitalize the transgenic pollen (23, 24), and the red fluorescence protein gene from Discosoma sp. (DsRed) (25) under the aleurone-specific LTP2 promoter (26) to mark the transgenic seed. Because OsNP1 is a sporophytic male fertility gene, a hemizygous OsNP1 transgene in the osnp1 mutant plant can fully restore the male fertility. Because ZM-AA1 driven by a PG47 promoter is a gametophytic factor that disrupts starch accumulation only in the transgenic pollen (23), only the transgenic pollen grains produced by the hemizygous transgenic plant are deactivated. The T0 transgenic plants were allowed to self-pollinate, and the T1 progeny was screened for the plant lacking the first T-DNA but carrying a single copy of the second T-DNA. The selected T1 plant was homozygous of the osnp1-1 locus but hemizygous of the second T-DNA, because pollen grains carrying the transgene were all defective, and the transgene was inherited only by the female gamete. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net