pCTCON2 Aga2P-HRP BioVector NTCC质粒载体菌种细胞基因保藏中心

pCTCON2 Aga2P-HRP BioVector NTCC质粒载体菌种细胞基因保藏中心 Biovector073152 pCTCON2 Aga2P-HRP AmpR.1vial,in E.coli. Storage:4℃ Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression. HRP = full-length horseradish peroxidase. This vector contains the following features: EcoRI-Aga2P-HA-TEVs-15 aa linker-NheI-HRP-3 aa linker-BamHI-myc-stop-XhoI GAL1 promoter (galactose inducible) 15 aa linker: GGGGSGGGGSGGGGS 3 aa linker: GSG HA: YPYDVPDYA myc: EQKLISEEDL TEVs: ENLYFQG, cleavage recognition site for TEV protease This construct is derived from the previously reported Aga2P-HRP construct (Lam et. al. Nature Methods, 12, 51-54, 2015), except the codon optimization of the synthetic HRP gene is different in this construct. • Vector backbone pCTCON2 • Backbone size w/o insert (bp)55791 • Total vector size (bp)7171 • Vector type Yeast Expression • Bacterial Resistance(s) Ampicillin • Growth Temperature 37°C • Growth Strain(s) DH5alpha • Copy number Unknown • Gene/Insert name Aga2P-HRP • Species S. cerevisiae (budding yeast), Synthetic • Insert Size (bp) 1380 • PromoterGAL1 • Tags / Fusion Proteins • HA tag • myc tag • Cloning methodRestriction Enzyme • 5′ cloning siteNheI (not destroyed) • 3′ cloning siteBamHI (not destroyed) • 5′ sequencing primergactacgctctgcaggagaacttg BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net