乳酸菌食品级表达载体pNZ8148,NZ9000分泌型质粒及乳酸杆菌菌株

The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.

The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.

pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).

序列Sequence：

AGATCTAGTCTTATAACTATACTGACAATAGAAACATTAACAAATCTAAAACAGTCTTAATTCTATCTTG

AGAAAGTATTGGTAATAATATTATTGTCGATAACGCGAGCATAATAAACGGCTCTGATTAAATTCTGAAG

TTTGTTAGATACAATGATTTCGTTCGAAGGAACTACAAAATAAATTATAAGGAGGCACTCACCATGGGTA

CTGCAGGCATGCGGTACCACTAGTTCTAGAGAGCTCAAGCTTTCTTTGAACCAAAATTAGAAAACCAAGG

CTTGAAACGTTCAATTGAAATGGCAATTAAACAAATTACAGCACGTGTTGCTTTGATTGATAGCCAAAAA

GCAGCAGTTGATAAAGCAATTACTGATATTGCTGAAAAATTGTAATTTATAAATAAAAATCACCTTTTAG

AGGTGGTTTTTTTATTTATAAATTATTCGTTTGATTTCGCTTTCGATAGAACAATCAAATCGTTTCTGAG

ACGTTTTAGCGTTTATTTCGTTTAGTTATCGGCATAATCGTTAAAACAGGCGTTATCGTAGCGTAAAAGC

CCTTGAGCGTAGCGTGGCTTTGCAGCGAAGATGTTGTCTGTTAGATTATGAAAGCCGATGACTGAATGAA

ATAATAAGCGCAGCGTCCTTCTATTTCGGTTGGAGGAGGCTCAAGGGAGTTTGAGGGAATGAAATTCCCT

CATGGGTTTGATTTTAAAAATTGCTTGCAATTTTGCCGAGCGGTAGCGCTGGAAAATTTTTGAAAAAAAT

TTGGAATTTGGAAAAAAATGGGGGGAAAGGAAGCGAATTTTGCTTCCGTACTACGACCCCCCATTAAGTG

CCGAGTGCCAATTTTTGTGCCAAAAACGCTCTATCCCAACTGGCTCAAGGGTTTGAGGGGTTTTTCAATC

GCCAACGAATCGCCAACGTTTTCGCCAACGTTTTTTATAAATCTATATTTAAGTAGCTTTATTTTTGTTT

TTATGATTACAAAGTGATACACTAATTTTATAAAATTATTTGATTGGAGTTTTTTAAATGGTGATTTCAG

AATCGAAAAAAAGAGTTATGATTTCTCTGACAAAAGAGCAAGATAAAAAATTAACAGATATGGCGAAACA

AAAAGATTTTTCAAAATCTGCGGTTGCGGCGTTAGCTATAGAAGAATATGCAAGAAAGGAATCAGAACAA

AAAAAATAAGCGAAAGCTCGCGTTTTTAGAAGGATACGAGTTTTCGCTACTTGTTTTTGATAAGGTAATT

ATATCATGGCTATTAAAAATACTAAAGCTAGAAATTTTGGATTTTTATTATATCCTGACTCAATTCCTAA

TGATTGGAAAGAAAAATTAGAGAGTTTGGGCGTATCTATGGCTGTCAGTCCTTTACACGATATGGACGAA

AAAAAAGATAAAGATACATGGAATAGTAGTGATGTTATACGAAATGGAAAGCACTATAAAAAACCACACT

ATCACGTTATATATATTGCACGAAATCCTGTAACAATAGAAAGCGTTAGGAACAAGATTAAGCGAAAATT

GGGGAATAGTTCAGTTGCTCATGTTGAGATACTTGATTATATCAAAGGTTCATATGAATATTTGACTCAT

GAATCAAAGGACGCTATTGCTAAGAATAAACATATATACGACAAAAAAGATATTTTGAACATTAATGATT

TTGATATTGACCGCTATATAACACTTGATGAAAGCCAAAAAAGAGAATTGAAGAATTTACTTTTAGATAT

AGTGGATGACTATAATTTGGTAAATACAAAAGATTTAATGGCTTTTATTCGCCTTAGGGGAGCGGAGTTT

GGAATTTTAAATACGAATGATGTAAAAGATATTGTTTCAACAAACTCTAGCGCCTTTAGATTATGGTTTG

AGGGCAATTATCAGTGTGGATATAGAGCAAGTTATGCAAAGGTTCTTGATGCTGAAACGGGGGAAATAAA

ATGACAAACAAAGAAAAAGAGTTATTTGCTGAAAATGAGGAATTAAAAAAAGAAATTAAGGACTTAAAAG

AGCGTATTGAAAGATACAGAGAAATGGAAGTTGAATTAAGTACAACAATAGATTTATTGAGAGGAGGGAT

TATTGAATAAATAAAAGCCCCCCTGACGAAAGTCGACGGCAATAGTTACCCTTATTATCAAGATAAGAAA

GAAAAGGATTTTTCGCTACGCTCAAATCCTTTAAAAAAACACAAAAGACCACATTTTTTAATGTGGTCTT

TTATTCTTCAACTAAAGCACCCATTAGTTCAACAAACGAAAATTGGATAAAGTGGGATATTTTTAAAATA

TATATTTATGTTACAGTAATATTGACTTTTAAAAAAGGATTGATTCTAATGAAGAAAGCAGACAAGTAAG

CCTCCTAAATTCACTTTAGATAAAAATTTAGGAGGCATATCAAATGAACTTTAATAAAATTGATTTAGAC

AATTGGAAGAGAAAAGAGATATTTAATCATTATTTGAACCAACAAACGACTTTTAGTATAACCACAGAAA

TTGATATTAGTGTTTTATACCGAAACATAAAACAAGAAGGATATAAATTTTACCCTGCATTTATTTTCTT

AGTGACAAGGGTGATAAACTCAAATACAGCTTTTAGAACTGGTTACAATAGCGACGGAGAGTTAGGTTAT

TGGGATAAGTTAGAGCCACTTTATACAATTTTTGATGGTGTATCTAAAACATTCTCTGGTATTTGGACTC

CTGTAAAGAATGACTTCAAAGAGTTTTATGATTTATACCTTTCTGATGTAGAGAAATATAATGGTTCGGG

GAAATTGTTTCCCAAAACACCTATACCTGAAAATGCTTTTTCTCTTTCTATTATTCCTTGGACTTCATTT

ACTGGGTTTAACTTAAATATCAATAATAATAGTAATTACCTTCTACCCATTATTACAGCAGGAAAATTCA

TTAATAAAGGTAATTCAATATATTTACCGCTATCTTTACAGGTACATCATTCTGTTTGTGATGGTTATCA

TGCTGGATTGTTTATGAACTCTATTCAGGAATTGTCAGATAGGCCTAATGACTGGCTTTTATAATATGAG

ATAATGCCGACTGTACTTTTTACAGTCGGTTTTCTAATGTCACTAACCTGCCCCGTTAGTTGAAGAAGGT

TTTTATATTACAGCTCC

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我们拥有目前最大的cDNA克隆库、覆盖30,000个人全长基因的TrueClone cDNA克隆和超过25,000个TrueORF cDNA克隆。利用TrueORF cDNA克隆，我们开发了大量的全长人重组蛋白产品（哺乳动物细胞表达），可以进行蛋白功能的研究。

标签：以下资源由Biovector Inc. 质粒载体菌株细胞株基因保藏中心独家供应并发布，侵权必究。Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com 质粒载体索取报价价格优惠售价出售图谱序列酶切位点转化构建求购购买订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site

Taq,Pfu高表达载体菌株；KOD,T4连接酶等高表达质粒载体,高产基因工程菌株、酶基因KOD,T4 kinase激酶，Tth反转录酶MMLV，Klentaq PCR酶，Cre酶，RNA inhibitor抑制酶，DL2000质粒

Gateway入门载体（gentamicin抗性）pDONR207，植物表达载体pJIT163-hGFP，pJIT166，植物双分子荧光互补(bimolecular fluorescence complementation，BiFC)载体pCCFP-X,pX-CCFP,pX-NYFP,pNYFP-X；

乳酸菌表达载体系列

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乳酸菌表达载体pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表达载体pVE5523，pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii

枯草芽孢杆菌表达载体系列

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pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01， 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,广宿主质粒pVLT33

腺病毒/慢病毒逆病毒载体系列

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腺病毒系统（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大肠杆菌BJ5183,293,293T cell line 腺相关病毒系统（Stratagene）： pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS， 慢病毒载体:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry质粒载体。 病毒包装：二质粒包装系统一： psPAX2 pMD2.G 二质粒系统二： pCMV-dR8.2 dvpr pCMV-VSVG 三质粒包装系统具有更高的包装效率： pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包装系统： PLP1 PLP2 PLP/VSVG

原核表达载体系列

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pQE系列pQE30,31,32,40,60,61,62, pGEX系列(含GST标签)，pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA(分泌型表达系列),pQBI63(原核表达带荧光),pET系列pET-GST,pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-),pINIIIompA,九载体原核表达试剂盒(高效、可溶、分泌、标签)pLLP-OmpA、pLLP-STII、pMBP-P、pMBP-C,pTrc-CKS,pET-GST、pET-Trx、pET-His,pET-DsbA,大肠杆菌冷激质粒： pColdI pColdII pColdIII pColdTF 原核共表达质粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大肠杆菌分子伴侣： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞： DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5a（pir） Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)

毕赤/酿酒酵母表达载体系列

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酿酒酵母表达载体pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,pRS41H,酵母载体pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF,酵母基因重组表达载体pUG6, pSH47,酵母单杂载体pHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统:酿酒酵母Y187,酿酒酵母AH109；质粒pGADT7,pGBKT7；对照质粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，酿酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 毕赤酵母表达载体pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套毕赤酵母Pichiapink,毕赤酵母宿主X33，KM71，KM71H，GS115,

植物表达/RNAi载体农杆菌系列

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pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi载体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入载体(随机突变体库)pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1

RNAi基因沉默干扰载体系列

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pSilencer1.0，pSilencer 2.1-U6 hygro， pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo， pSilencer 4.1-CMV neo， pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi载体（oligoengine） pSuper-puro RNAi逆转录病毒载体(clontech): RNAi-Ready pSIREN-Retro Q， RNAi-Ready pSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide） RNAi慢病毒载体: pLKO.1

基因cDNA文库ORF表达载体系列

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拥有目前最大的cDNA克隆库、覆盖10万人、鼠全长基因的cDNA克隆和超过25,000个ORF克隆表达载体。药物转运体基因蛋白表达细胞株：肝脏 P-gp/MDR1 OAT2 OAT7 1-Oct OATP8 (OATP1B3) OATP-B (OATP2B1) OATP2（OATP1B1) 肾脏 P-gp/MDR1 OAT1 OAT2 OAT3 OAT4 2-Oct URAT1 PEPT1 PEPT2 OCTN1 OCTN2 小肠 P-gp/MDR1 1-Oct PEPT1 OATP 脑血屏障 P-gp/MDR1 BCRP OATP-A (OATP1A2) OATP-B (OATP2B1)

哺乳动物表达,GS系统

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谷氨酰胺合成酶加压筛选表达系统GS expression system vectors pEE12.4, pEE6.4, 配套CHOK1SV细胞株，GS表达载体pGP3, pGP3Due, pGP3Con, 配套cGMP banking record cell line，DHFR筛选表达系统载体pSV2-DHFR，pCI-DHFR, pcDNA4-DHFR, pGP2, pGP2Due, pGP2Con, 疫苗/抗体表达载体pCEP4,细胞高表达载体pTT5,配套细胞株HEK293E, 哺乳动物表达载体pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1(-)/myc-His A ，pcDNA6-Myc/His B， pCEP4， pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II哺乳动物诱导表达系统:pOPRSVI ，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳动物表面展示系统:pDisplay, 四环素调控系统（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四环素调控系统（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTet-off信号通路报告载体:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]，pGL4.75;p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系统（invitrogen）pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR

热门日志

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绿色荧光pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1，pAcGFP1-1, pEGFP-Actin,  pIRES2-EGFP,pCMV/myc/Nuc,pCMV/myc/Nuc-GFP， pLEGFP-C1，pLEGFP-N1，青色荧光pECFP-N1，pECFP-C1，pECFP-ER由Biovector公司现货供应

W3110 strain菌株现货供应-Biovector质粒菌株保藏中心

Biovector公司pCDH-EF1-MCS-T2A-Puro pLVTHM pLOX-CW-CRE pP-αSUMO3 SUMO protease 2菌株 PiggyBac转座子系统Super PiggyBac Transposase (PB200PA-1)  PiggyBac Dual promoter (PB513B-1)  PiggyBac 3’LTR (pMD18T pCDH-EF1-MCS-T2A-Puro pBI CMV1 BL21gold(DE3) plysS菌株 pET-SUMO

Biovector公司pCDH-EF1-MCS-T2A-Puro  pLVTHM  pLOX-CW-CRE  pP-αSUMO3  SUMO protease 2菌株 pCDH-EF1-MCS-T2A-Puro  pBI CMV1  BL21gold(DE3) plysS菌株 pET-SUMO

pIRES2-DsRed， pmCherry-N1，pmCherry-C1，黄色荧光pEYFP-C1, pEYFP-N1, pEYFP-Golgi,  pEYFP-Mem,  pEYFP-ER , pEYFP actin，pEYFP tub由Biovector公司现货供应

Biovector公司pLOX-Ttag-iresTK  pLOX-TERT-iresTK  pLOX-CWBmi1  pFP93  pCDF1-MCS2-EF1-copGFP  pLentG-KOSM  TetO-FUW-OSKM  FUW-M2rtTA pCDH-CMV-MCS-EF1-GFP+Puro (pCD513B-1)  pWPXL  pCDH-CMV-MCS-EF1-copGFP

Biovector公司供应ExAssist interference-resistant helper phage EZ10 FOPFlash   XL1-Blue MRF’ XL2-Blue MRF’ XLOLR Y187 Y2H Gold Yeast strain FT-T FT-T Simple FUGW FUW-M2rtTA FUW-tetO-hKLF4 FUW-tetO-hMYC FUW-tetO-hOCT4 FUW-tetO-hSOX2 GM2163 GS115 HA-mPB HB2151 H

Biovector公司pHSE-Luc  pGRE-Luc  pCRE-Luc  pAP1(PMA)-TA-Luc  pCaspase3-sensor  pIκB-EGFP  PiggyBac转座子 系统Super PiggyBac Transposase (PB200PA-1)  PiggyBac Dual promoter (PB513B-1)  PiggyBac 3’LTR (pMD18T vector)  PiggyBac 5’LTR (pMD18T vector)  HA-mPB

广宿主载体系列假单胞菌链球菌表达载体

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pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 转座子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1

基因重组敲除/自杀质粒载体系列

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pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革兰氏阳性菌基因敲除)，pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶载体PL253，PL451，PL452，PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374

荧光蛋白表达载体

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pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen，pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc，pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1，pLEGFP-N1，pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 启动子增强子报告载体（Promega，带有Luc荧光报告基因）： pGL3-basic，pGL3-control，pGL3-enhancer，pGL3-promoter，pRL-TK，pRL-CMV，pRL-SV40, 信号通路报告载体（Clontech）： pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP， pNFAT-TA-Luc，pCaspase3-sensor，pAP1(PMA)-Luc 信号通路报告载体（Promega）： pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]，pGL4.75 信号通路报告载体（Stratagene）： p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc， pFA2-Elk1，pFC-MEKK，pFR-luc

昆虫/杆状病毒表达载体

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pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大肠杆菌,pMIB v5-His B ,pIZT/V5-His

最新日志

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pX330  pX335  hCas9  gRNA_CloningVector  pX260 pX334  hCas9_D10A  MLM3613  DR274  pMD2.G Golden Gate TALEN Kit 2.0  TALE Toolbox  TALEN Accessory Pack  LIC TAL Effector Assembly Kit  CCSB-Broad Human Kinase ORF Collection由Biovector Inc.现货供应

EL350 EL350 (galk+) EL350 (galk-) WB800 ATCC 13032 BL21（DE3） 2T1R(CCDB) JM109 DH10B DH-5α 由质粒载体菌株细胞株基因保藏中心现货供应

pMXS-puro pMXS-puro-luciferase pMXS-puro-H2B-eGFP plko.1-IRES-GFP plko.1-EGFP plko.1-H2B-GFP plko.1-linker plko.1-CMV plko.1-trc Lactobacillus sake CGMCC 1.2244 CGMCC1.1215 ATCC 13032 WB800 2T1R(CCDB) BL21（DE3） EL350 EL350 (galk+) EL350 (galk-)由普如汀公司现货供应

pCDNA3.1-Home-ERK2 pcDNA3.1 pWB980 pET-22b-sit pBV220 pBV220-gam-bet-txo pXMJ-19 pENTR gus pEF-EntrB pENTR-3c-IRES-GFP pEntr-3c-Home-ERK1 pMXS-IRES-GFP pDEST 8 pDEST 27 pcDNA DEST-40 pcDNA DEST-47 pcDNA DEST-53 plko.1-RFPuro plko.1-d2eGFP由质粒菌株库现货供应

pCAG-Cre-GFP pCAG-Cre pCAG-Cre-ERT2 pGIPZ-akt1 pFD pBFP-IRES-puro pLC3-CFP pLamp1-cherry red pLamp1-YFP p3XFlag-CMV-7.1-Homo-MKL1 pSG5-Homo-SRCIa pEGFP-IRES-neo pEYFP-N1 pEGFP-C3 pRK5-Home-smad3 pRK5-Home-smad2 pCDNA3.1-Home-ER α 由质粒载体菌株细胞株基因保藏中心现货供应

pCDNA3.1-Mouse-MRTF-A pCDNA3.1-Mouse-myocardin pCDNA3.1-Mouse-stat3F pEF1 pBS-MDa pPK2 pCMV-GAG-pol pDEST 12.2 pTranslucent pSJ4 pANGH3 pGL4.74hRluc/TK ptTs-NEO pQC-tTS-IN pSIREN-retorQ pSinoa pGL3-Basic pAmpho pFD-NTC phelp pECO p10A1由质粒菌株库现货供应

BL21 SI BL21 Star (DE3) C600 DH5α GI698 GI724 HB2151 JF1125 JM105 JM109 JM109(DE3) K802 LMG194 YK537 GS115 GS115β-Gal GS115 HisA菌株由Biovector公司现货供应

W3110 strain菌株现货供应-Biovector质粒菌株保藏中心

BiFC双分子荧光质粒

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pBiFC-VC155，pBiFC-VN173，pBiFC-bFosVC155，pBiFC-bFos(deltaZIP)VC155，pBiFC-bjun-VN173，pCE-BiFC-VN173，pCE-BiFC-VC155

真菌/黑、黄曲霉表达载体

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pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT农杆菌介导转化载体 pBHt1,pBIG2RHPH2-GFP-GUS等

酵母单杂/双杂/三杂交载体系列

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双杂交系统pGBKT7，pGADT7，pCL1，pGBKT7-53，pGADT7-T，pGBKT7-Lam，配套酿酒酵母AH109 酵母单杂交系统（Clontech）:pHISi,pLacZi,pHis2,pHIS2.1 酵母三杂交系统:pBridge, YBZ1菌株 Cytotrap Two-Hybrid System（Stratagene）: pSos, pSos MAFB, pMyr

噬菌体展示系统

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1.噬菌体展示抗体scFv 质粒：pCANTAB5E 宿主菌：TG1 辅助噬菌体：M13K07 可溶表达菌：HB2151 2.噬菌体展示抗体Fab 质粒：pcomb3 宿主菌：XL1-Blue 辅助噬菌体：VCSM13千余种细胞株：CHOK1-SV，HEK293T,FT,HEK293E,HEK293-6E,Sf9,肺癌细胞，乳腺癌细胞，猴肾细胞，前列腺癌细胞，卵巢细胞，肝癌细胞，食管癌细胞