| JAWSII |
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| ZymoGenetics, Inc. |
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| Biosafety Level: | 1 |
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| frozen |
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| Medium & Serum: | See Propagation |
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| mixed, adherent and suspension |
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| Mus musculus |
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| monocyte
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| Organ: bone marrow
Strain: C57BL/6
Cell Type: immature dendritic cell; monocyte; |
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| In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| Propagation: | ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%
Temperature: 37.0°C |
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| Protocol: Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week |
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| Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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| recommended serum:ATCC 30-2020 |
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| 38868: MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997
47440: Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998 |
