HEK293T-CRISPRa人类转化细胞株NTCC® Human Transformed cell line-BioVector NTCC细胞保藏中心CVCL_C9DX

[Designation名称] NTCC® HEK293T-CRISPRa Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Cross references: BioGRID_ORCS_Cell_line; 1656
Cross references: Wikidata; Q123031592
Reference Identifiers: PubMed=34431042;
Comments: Characteristics: Transduced with lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1_Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) (PubMed=34431042).
Comments: Genetic integration: Method=Transduction; Gene=HGNC; 5224; HSF1.
Comments: Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368 del).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession: CVCL_C9DX
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® HEK293T-CRISPRa Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net