KG-1-C Cell Line人神经胶质瘤细胞株-BioVector NTCC典型培养物保藏中心RCB0270

Name：KG-1-C

Profile

mixed glioma, GFAP-negative, S-100-positive (growth is very slow).

Other Name

Animal

human

Strain

Genus

Homo

Species

sapiens

Sex

M

Age

13

Identity

available

Tissue for Primary Cancer

neural

Case history

Metastasis

Tissue Metastasized

Genetics

mixed glioma

Life Span

Crisis PDL

Morphology

Character

mixed glioma

Classify

Established by

Miyake,E.

Comment

Year

1987

Medium

Dulbecco's modified Eagle's medium with 10% fetal calf serum

Methods for Passages

200 units trypsin, 0.05 % EDTA

Cell Number on Passage

5x10^4 cells/ml

Race

CO2 Conc.

5 %

Tissue Sampling

brain

Tissue Type

Cell Density at Seeding

1.0 - 6.8 x 10^4 cells/mL

Methods for Passages

Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.

Doubling Time

Very slow (D.T.=3.5 - 5 days)

Cell Number in Vial (cells/1ml)

9.0 x 10^5

Viability at cell freezing (%)

94.8

Antibiotics Used

free

Detection of virus genome fragment by Real-time PCR

Detected DNA Virus

tested

Detected RNA Virus

tested

CMV

-

parvoB19

-

HCV

-

HTLV-1

-

EBV

-

HBV

-

HIV-1

-

HTLV-2

-

HHV6

-

HTLV-1

-

HIV-2

-

HAV

-

HHV7

-

HTLV-2

-

-/negative.

+/positive.

nt/not tested.

(positive (+) does not immediately mean the production of infectious viral particles.)

BKV

-

HIV-1

-

JCV

-

HIV-2

-

ADV

-

HPV18

-