Fluorescent Proteins: Subcellular Localization plasmids vectors亚细胞定位质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Fluorescent Proteins: Subcellular Localization plasmids vectors亚细胞定位质粒载体<br/></br>Background

The subcellular localization of a protein is often tied to its function, so it's important to determine where your protein of interest resides. There are a variety of methods for determining subcellular localization. One of the most common is to use fluorescence microscopy to compare the localization of your protein of interest with known markers.</br>BioVector has assembled a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess whether your protein is targeted to the same structures as these well-characterized proteins.</br>

General Structures

The cell contains a variety of organelles and subcellular structures each with their own unique function. The nucleus, for example, houses the cell’s genetic material. The mitochondria produces the energy cells required for chemical reactions. Labeling these structures can be quite useful for identifying the localization or possible function of a protein of interest. Browse or search the table below to find markers of general cellular organelles and structures.</br>Showing 1 to 56 of 56 entries

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Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI

CAV1-mCherry Caveolae Cav1 mCherry Helenius

pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez

H2B-mCherry Chromatin H2B mCherry Benezra

H2B-GFP Chromatin H2B GFP Wahl

GFP-HP1gamma Chromatin HP1 gamma GFP Tom Misteli

pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich

PGK-H2BeGFP Chromatin H2B EGFP Mark Mercola

PGK-H2BmCherry Chromatin H2B mCherry Mark Mercola

pHIV-H2BmRFP Chromatin H2B mRFP Bryan Welm, Zena Werb

LV-GFP Chromatin H2B GFP Elaine Fuchs

pH2B-miRFP703 Chromatin H2B miRFP703 Verkhusha

pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez

cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Wada

pCAG-mGFP Membrane palmitoylation sequence from GAP43 EGFP Cepko

pPalmitoyl-mTurquoise2 Membrane palmitoylation sequence from p63 mTurquoise2 Gadella

Lck-GFP Membrane palmitoylation sequence from Lck EGFP Green

pQC membrane TdTomato IX Membrane Palmitoylation TdTomato Connie Cepko

FUmGW Membrane Palmitoylation GFP Connie Cepko

pmTurquoise2-Mito Mitochondria COX8A(1-29) mTurquoise2 Gadella

mCherry-Mito-7 Mitochondria Mitochondrialtargeting sequence(COX8A) mCherry* Davidson

mito-BFP Mitochondria mitochondrial targeting signal (COX4) TagBFP Voeltz

pLV-mitoGFP Mitochondria COX8 GFP Pantelis Tsoulfas

mCherry-Parkin Mitochondria Park2 mCherry Richard Youle

pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha

pABCb10-GFP Mitochondria ABCb10 GFP Shirihai

pMito-miRFP703 Mitochondria COX8A miRFP703 Verkhusha

pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez

pCMV-mGold-Mito-N-7 Mitochondria Mito mGold Francois St-Pierre

GFP-Mff Mitochondria-Outer Membrane Mff acGFP1 Voeltz

mCherry-TOMM20-N-10 Mitochondria-Outer Membrane TOMM20 mCherry* Davidson

pmTurquoise2-NES Non-nucleus Nuclear Export Sequence mTurquoise2 Gadella

pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet Gadella

pmScarlet-i_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet-I Gadella

pmScarlet-H_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet-H Gadella

mCherry-LaminB1-10 Nuclear Envelope LaminB1 mCherry* Davidson

mCherry-LaminA-C-18 Nuclear Envelope LaminA1 mCherry* Davidson

pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Huang

pEBFP2-Nuc Nucleus NLS EBFP2 Campbell

pmTurquoise2-H2A Nucleus H2A mTurquoise2 Gadella

pQC NLS mCherry IX Nucleus NLS mCherry Cepko

pEGFP-SF2 Nucleus SF2 EGFP Tom Misteli

pmKalama1-Nuc Nucleus NLS mKamala1 Campbell

pmScarlet_H2A_C1 Nucleus H2A mScarlet Gadella

pQC NLS YFP IX Nucleus NLS YFP Cepko

pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Gadella

pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p protein) dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez

pmScarlet-H_H2A_C1 Nucleus H2A mScarlet-H Gadella

pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre

pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-Pierre

pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Dammeyer

pmScarlet_peroxisome_C1 Peroxisome SRL mScarlet Gadella

pmScarlet-I_peroxisome_C1 Peroxisome SRL mScarlet-I Gadella

pmScarlet-H_peroxisome_C1 Peroxisome SRL mScarlet-H Gadella

pmTurquoise2-Peroxi Peroxisomes Peroxisomal Targeting Sequence mTurquoise2 Gadella

mCherry-Peroxisomes-2 Peroxisomes Peroximal Targeting Signal 1 (PTS1) mCherry* Davidson

pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisomes SKL tripeptide, peroxisome transport signal dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.</br>Return to top</br>Cytoskeletal Elements

The cytoskeleton is the cell’s internal framework that assists in sustaining structure and function. The cytoskeleton is primarily made up of microtubules, actin filaments, and intermediate filaments. These structures give the cell its shape, organize cellular organelles, and are necessary for movement and cell division. Browse or search the table below to find markers of cytoskeletal element.</br>Showing 1 to 44 of 44 entries

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Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI

GFP-cortactin Actin Filaments Cortactin EGFP Anna Huttenlocher

pEGFP-N1 alpha-actinin 1 Actin Filaments alpha-actinin 1 EGFP Carol Otey

Cortactin-pmCherryC1 Actin Filaments Cortactin mCherry Christien Merrifield

pLifeAct-mTurquoise2 Actin Filaments LifeAct mTurquoise2 Dorus Gadella

pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella

pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins

mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson

mEGFP-Lifeact-7 Actin Filaments LifeAct EGFP Michael Davidson

eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer

pDEST/LIfeAct-mCherry-N1 Actin Filaments LifeAct mCherry Robin Shaw

pCAG-mGFP-Actin Actin Filaments beta-actin EGFP Ryohei Yasuda

pPAmCherry-b-actin Actin Filaments beta-actin PAmCherry1 Vladislav Verkhusha

GFP-UtrCH Actin Filaments utrophin (aa# 1-261) GFP William Bement

mCherry-UtrCH Actin Filaments Utrophin mCherry William Bement

pLifeAct_mScarlet-i_N1 Actin Filaments LifeAct mScarlet-I Dorus Gadella

pLifeAct-miRFP703 Actin Filaments LifeAct miRFP703 Vladislav Verkhusha

pLifeAct_mScarlet-H_N1 Actin Filaments LifeAct mScarlet-H Dorus Gadella

pCMV-mGold-Actin-C-18 Actin Filaments Actin mGold Francois St-Pierre

GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru

pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg

pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg

pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg

mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson

pKeratin-miRFP703 Intermediate Filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha

pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown

pEGFP-rNFM Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown

pVimentin-miRFP703 Intermediate Filaments (vimentin) vimentin miRFP703 Vladislav Verkhusha

pmTurquoise2-Tubulin Microtubules alpha-tubulin mTurquoise2 Dorus Gadella

mCh-alpha-tubulin Microtubules alpha-tubulin mCherry Gia Voeltz

pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin EGFP Patricia Wadsworth

EMTB-3XGFP Microtubules ensconsin GFP William Bement

EMTB-mCherry Microtubules ensconsin mCherry William Bement

pmScarlet_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet Dorus Gadella

mCherry-Tubulin-C-18 Microtubules alpha-tubulin mCherry* Michael Davidson

pPAmCherry-a-tubulin Microtubules alpha-tubulin PAmCherry Vladislav Verkhusha

pmScarlet-i_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-I Dorus Gadella

pmScarlet-H_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-H Dorus Gadella

pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre

pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru

pDEST/N1-hEB1-GFP Microtubules EB1 GFP Robin Shaw

pmiRFP703-Tubulin Microtubules alpha-tubulin miRFP703 Vladislav Verkhusha

pEB3-miRFP703 Microtubules EB3 miRFP703 Vladislav Verkhusha

GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru

pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.</br>Return to top</br>Cell Junctions

Cell junctions are responsible for forming connections and adhesions between animal cells. They are important for holding cell structures together and reducing cell stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers for cellular junctions.</br>Showing 1 to 9 of 9 entries

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Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI

E-cadherin-GFP Adherens junctions E-cadherin GFP Stow

Paxillin-pEGFP Focal adhesions paxillin GFP Horwitz

pPaxillin-PSmOrange Focal adhesions paxillin PSmOrange Verkhusha

mEmerald-Paxillin-22 Focal Adhesions paxillin mEmerald* Davidson

pZyxin-miRFP703 Focal adhesions Zyxin miRFP703 Verkhusha

pDEST/hCx43-EGFP-N1 Gap Junctions Connexin 43 EGFP Shaw

pDEST-mCherry-GJA1-20k-N1 Gap Junctions Connexin 43 / GJA1-20k mCherry Shaw

mCherry-Cx43-7 Gap Junctions Connexin 43 / GJA1-20k mCherry* Davidson

mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Davidson

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.</br>Return to top</br>Secretory Pathway

The secretory pathway is named for its role in secreting proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding or post-translational modification to function properly. Browse or search the table below to find markers of secretory pathway components.</br>Showing 1 to 48 of 48 entries

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Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI

pEGFP-LC3 (human) Autophagosome LC3 EGFP Finkel

pCMB-AUTr Autophagosome LC3(ATG8) mCherry Harrison

mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Helenius

Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Zhuang

mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Davidson

pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin miRFP703 Verkhusha

DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Pagano

mRFP-Rab5 Early endosomes RAB5A mRFP Helenius

mCh-Rab5 Early endosomes Rab5 mCherry Voeltz

BFP-Rab5 Early endosomes Rab5 BFP Voeltz

GFP-Rab5B Early endosomes Rab5B AcGFP Voeltz

GFP-Rab4B Early endosomes Rab4B AcGFP Voeltz

pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP Johnson

pTag-BFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagBFP Johnson

pEF.myc.ER-E2-Crimson Endoplasmic Reticulum ER retention signal E2-Crimson Glick

pmTurquoise2-ER Endoplasmic Reticulum ER retention signal mTurquoise2 Gadella

mCh-Sec61 beta Endoplasmic Reticulum Sec61 mCherry Voeltz

BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Voeltz

BFP-KDEL Endoplasmic Reticulum BiP ER signal sequence BFP Voeltz

Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP Voeltz

ERmoxGFP Endoplasmic Reticulum ER retention signal moxGFP Snapp

ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Snapp

pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic reticulum (ER) KDEL dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez

pCytERM_mScarlet-i_N1 ER CytERM mScarlet-I Gadella

pCytERM_mScarlet-H_N1 ER CytERM mScarlet-H Gadella

pCytERM_mScarlet_N1 ER CytERM mScarlet Gadella

pmTurquoise2-Golgi Golgi B4GALT1(1-61) mTurquoise2 Gadella

GolgiCFP Golgi eNOS(1-33) CFP Newton

GalToxBFP Golgi GalT signal anchor transmembrane domain oxBFP Snapp

pmScarlet-i_Giantin_C1 Golgi apparatus Giantin mScarlet-I Gadella

pmScarlet-H_Giantin_C1 Golgi apparatus Giantin mScarlet-H Gadella

pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet Gadella

GFP-rab7 WT Late endosomes RAB7 GFP Pagano

DsRed-rab7 WT Late endosomes RAB7 DsRed Pagano

mCh-Rab7A Late endosomes Rab7a mCherry Voeltz

GFP-Rab7A Late endosomes Rab7a AcGFP Voeltz

pTag-RFP-C-h-Rab7-c-Myc Late endosomes Rab7 TagRFP Johnson

pTag-BFP-C-h-Rab7-c-Myc Late endosomes Rab7 TagBFP Johnson

Lamp1-YFP Lysosomes Lamp-1 YFP Mothes

Lamp1-RFP Lysosomes Lamp-1 RFP Mothes

mCherry-Lysosomes-20 Lysosomes LAMP1 mCherry* Davidson

pLAMP1-miRFP703 Lysosomes LAMP1 miRFP703 Verkhusha

GFP-rab11 WT Recycling endosomes RAB11A GFP Pagano

DsRed-rab11 WT Recycling endosomes RAB11A DsRed Pagano

pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP Johnson

pTag-BFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagBFP Johnson

pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP Johnson

pTag-BFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagBFP Johnson

*Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.<br/></br>Supplier来源:BioVector NTCC Inc.<br/>TEL电话:+86-010-53513060<br/>Website网址: http://www.biovector.net