DescriptionBack to Top

Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl₂. All that is required is the addition of template, primers and water.

Product Source

Reagents Supplied

The following reagents are supplied with this product:

Advantages and FeaturesBack to Top

Features

• Robust Reactions - Maximal success with minimal optimization.

• Extreme Fidelity - > 50x greater than Taq

• High Speed - Dramatically reduced extension times (10x faster than Pfu)

• High Yield - Increased product yield using minimal amount of enzyme.

• Versatile - Can be used for routine per as well as long or difficult templates.

Applications

• PCR

• Cloning

• Long or Difficult Amplification

• High-Throughput PCR

Properties and UsageBack to Top

Storage Temperature

Heat Inactivation

Unit Assay Conditions

Related ProductsBack to Top

Companion Products

• Deoxynucleotide (dNTP) Solution Mix

• Deoxynucleotide (dNTP) Solution Set

• Detergent-free Phusion® GC Buffer Pack

• Detergent-free Phusion® HF Buffer Pack

• Magnesium Chloride (MgCl₂) Solution

• Phusion® GC Buffer Pack

• Phusion® HF Buffer Pack

• Phusion® High-Fidelity DNA Polymerase

• Phusion® High-Fidelity PCR Kit

• Phusion® High-Fidelity PCR Master Mix with GC Buffer

NotesBack to Top

1. Products specifications for individual components in the Phusion High-Fidelity PCR Master Mix with HF Buffer are available separately.

ReferencesBack to Top

1. Frey, B. and Suppman, B. (1995). Biochemica. 2, 34-35.

2. Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.