菌种 - Biovector质粒载体菌种细胞蛋白抗体基因保藏中心-NTCC典型培养物保藏中心

TK2420 is a mutant strain of Escherichia coli that is deficient in the Kdp, Trk, and Kup K+ uptake systems: K+ requirement TK2420 requires exogenous K+ to maintain steady-state pH homeostasis and to recover from sudden acid shifts. Complementation The recombinant plasmid pGerN, which expresses the endospore germination protein GerN, can complement the phenotype of TK2420. This suggests that GerN may catalyze both Na+ extrusion and K+ uptake. Growth inhibition Aa-KefB and Aa-NhaP inhibit the growth of TK2420, suggesting that they catalyze K+ efflux. K+ uptake K+ uptake by TK2420 yggS-T/pUT is slightly decreased by the addition of 5mM NaCl. Osmolarity The K+ requirement of TK2420 is partly compensated by supplementation with NaCl, choline chloride, proline, or sucrose. This suggests that the K+ requirement is mediated in part by osmolarity

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2024-11-12

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Lasiodiplodia iranensis NTCC®产茉莉酮真菌菌株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Lasiodiplodia iranensis产茉莉酮真菌菌株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心Jasmonic acid is a plant hormone...

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2024-11-12

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E. coli K-12 MG1655 NTCC®大肠杆菌专利菌株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E. coli K-12 MG1655 Description Genotype: F- lambda- ilvG- rfb-50 rph-1 Serotype: OR:H48:K- This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." [1]. The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil [2]. The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II [3]. The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis [4]. MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 [5]. References: Blattner, et al (1997) The complete genome sequence of Escherichia coli K-12. Science 277(5331), 1453-1462. PMID: 9278503 Jensen (1993) The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J Bacteriol 175(11), 3401-3407. PMID: 8501045 Lawther, et al. (1982) DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J Bacteriol 149(1), 294-298. PMID: 7033211 Liu and Reeves (1994) Escherichia coli K12 regains its O antigen. Microbiology 140(Pt 1), 49-57. PMID: 7512872 Bachmann (1996) Derivations and Genotypes of Some Mutant Derivatives of Escherichia coli K-12. p2460-2488 in Neidhardt (ed), Escherichia coli and Salmonella, 2nd Edition ASM Press, Washington DC Media and growth curves MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine). In doing experiments with microarrays, we sought a medium that was both defined and reproducible (unlike LB), yet supported fast growth rates. We now use Neidhardt's MOPS-based rich defined medium (MOPS-RDM). We also sought a commercially available rich defined medium, and finding none tried to grow MG1655 on a medium sold for mammalian cell culture. It grows quite well, but we decided to stick with Neidhardt's medium because it shows a sharper transition to stationary phase. Recipe for MOPS Rich Defined Medium and MOPS Minimal Medium Growth curve for MG1655 on MOPS Minimal Medium Information and growth curve for a commercially available rich defined medium (MEM alpha w/o phenol red) Growth curves for MG1655 on M9 Minimal Medium

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2024-11-18

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Azotobacter chroococcum CBD15圆褐固氮菌菌种-可产生吲哚乙酸植物生长激素-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

NTCC® Azotobacter chroococcum CBD15 is a strain of bacteria that was isolated from soil and characterized for its ability to fix atmospheric nitrogen and produce plant growth hormones: Nitrogen fixation Azotobacter chroococcum CBD15 is able to fix atmospheric nitrogen, converting it into forms that plants can use. Plant growth hormones Azotobacter chroococcum CBD15 produces indole acetic acid (IAA), a plant growth hormone. Eco-friendly Azotobacter chroococcum CBD15 can reduce graphene oxide (GO) to reduced graphene oxide (rGO) at room temperature. Azotobacter chroococcum CBD15 was used to create an engineered strain called Azotobacter chroococcum HKD15. The HKD15 strain was created by inoculating wheat seeds with the CBD15 strain. Azotobacter is a nitrogen-fixing bacteria that is aerobic, meaning it can only live in the presence of oxygen. Azotobacter species are found in neutral and weakly basic soils, and even in the Arctic and Antarctic. They can help crops grow, especially in soils that are polluted with heavy metals.

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2024-11-18

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Azotobacter chroococcum W5圆褐固氮菌菌种-可产生吲哚乙酸植物生长激素-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Azotobacter chroococcum W5圆褐固氮菌菌种-可产生吲哚乙酸植物生长激素BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心Azotobacte...

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2024-11-18

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铜绿假单胞菌NTCC® CCUG 60818 Pseudomonas aeruginosa -BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

铜绿假单胞菌NTCC® CCUG 60818 Pseudomonas aeruginosa -BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心Strain DetailsSampl...