DLG2 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

DLG2蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104969 [Species物种来源]: Homo sapiens 【 DLG2蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ DLG2 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 DLG2 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens DLG2 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 DLG2 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens DLG2 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 DLG2 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 DLG2 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104971 [Species物种来源]: Homo sapiens 【 HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HSD-40,C11ORF36,FLJ36102,MGC132631,MGC132633 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

P42,CDKL1,KKIALRE Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

P42,CDKL1,KKIALRE蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104977 [Species物种来源]: Homo sapiens 【 P42,CDKL1,KKIALRE蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ P42,CDKL1,KKIALRE Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 P42,CDKL1,KKIALRE Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens P42,CDKL1,KKIALRE protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 P42,CDKL1,KKIALRE Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens P42,CDKL1,KKIALRE [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 P42,CDKL1,KKIALRE Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 P42,CDKL1,KKIALRE Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

C14ORF45,FLJ23189 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

C14ORF45,FLJ23189蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104979 [Species物种来源]: Homo sapiens 【 C14ORF45,FLJ23189蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C14ORF45,FLJ23189 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C14ORF45,FLJ23189 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C14ORF45,FLJ23189 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C14ORF45,FLJ23189 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens C14ORF45,FLJ23189 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C14ORF45,FLJ23189 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C14ORF45,FLJ23189 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

BMF Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

BMF蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104983 [Species物种来源]: Homo sapiens 【 BMF蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ BMF Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 BMF Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens BMF protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 BMF Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens BMF [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 BMF Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 BMF Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

RAB36 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

RAB36蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104989 [Species物种来源]: Homo sapiens 【 RAB36蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RAB36 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RAB36 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RAB36 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RAB36 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens RAB36 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RAB36 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RAB36 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104999 [Species物种来源]: Homo sapiens 【 FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FHH,FIH,HHC,CASR,HHC1,NSHPT,PCAR1,GPRC2A,MGC138441 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

NXF,NPAS4,LE-PAS,PASD10 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

NXF,NPAS4,LE-PAS,PASD10蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC105001 [Species物种来源]: Homo sapiens 【 NXF,NPAS4,LE-PAS,PASD10蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NXF,NPAS4,LE-PAS,PASD10 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NXF,NPAS4,LE-PAS,PASD10 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NXF,NPAS4,LE-PAS,PASD10 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NXF,NPAS4,LE-PAS,PASD10 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens NXF,NPAS4,LE-PAS,PASD10 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NXF,NPAS4,LE-PAS,PASD10 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NXF,NPAS4,LE-PAS,PASD10 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

DGK,DGKB,DAGK2,DGK-BETA,KIAA0718蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC105005 [Species物种来源]: Homo sapiens 【 DGK,DGKB,DAGK2,DGK-BETA,KIAA0718蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 DGK,DGKB,DAGK2,DGK-BETA,KIAA0718 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

HNF4G,NR2A2 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

HNF4G,NR2A2蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC105009 [Species物种来源]: Homo sapiens 【 HNF4G,NR2A2蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HNF4G,NR2A2 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HNF4G,NR2A2 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HNF4G,NR2A2 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HNF4G,NR2A2 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens HNF4G,NR2A2 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HNF4G,NR2A2 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HNF4G,NR2A2 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector