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VMD2L2,MGC126872 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
VMD2L2,MGC126872蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC101823 [Species物种来源]: Homo sapiens 【 VMD2L2,MGC126872蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ VMD2L2,MGC126872 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 VMD2L2,MGC126872 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens VMD2L2,MGC126872 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 VMD2L2,MGC126872 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens VMD2L2,MGC126872 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 VMD2L2,MGC126872 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 VMD2L2,MGC126872 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
GBA3,GLUC,CBGL1,MGC104276,MGC126878蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC101829 [Species物种来源]: Homo sapiens 【 GBA3,GLUC,CBGL1,MGC104276,MGC126878蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ GBA3,GLUC,CBGL1,MGC104276,MGC126878 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 GBA3,GLUC,CBGL1,MGC104276,MGC126878 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens GBA3,GLUC,CBGL1,MGC104276,MGC126878 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 GBA3,GLUC,CBGL1,MGC104276,MGC126878 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens GBA3,GLUC,CBGL1,MGC104276,MGC126878 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 GBA3,GLUC,CBGL1,MGC104276,MGC126878 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 GBA3,GLUC,CBGL1,MGC104276,MGC126878 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC101837 [Species物种来源]: Homo sapiens 【 H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 H3T,H3.4,H3/G,H3FT,HIST3H3,MGC126886,MGC126888 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
OT,OXT,OT-NPI,MGC126890,MGC126892蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC101841 [Species物种来源]: Homo sapiens 【 OT,OXT,OT-NPI,MGC126890,MGC126892蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ OT,OXT,OT-NPI,MGC126890,MGC126892 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 OT,OXT,OT-NPI,MGC126890,MGC126892 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens OT,OXT,OT-NPI,MGC126890,MGC126892 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 OT,OXT,OT-NPI,MGC126890,MGC126892 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens OT,OXT,OT-NPI,MGC126890,MGC126892 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 OT,OXT,OT-NPI,MGC126890,MGC126892 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 OT,OXT,OT-NPI,MGC126890,MGC126892 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
KGFLP1,MGC126891 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
KGFLP1,MGC126891蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC101842 [Species物种来源]: Homo sapiens 【 KGFLP1,MGC126891蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ KGFLP1,MGC126891 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 KGFLP1,MGC126891 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens KGFLP1,MGC126891 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 KGFLP1,MGC126891 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens KGFLP1,MGC126891 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 KGFLP1,MGC126891 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 KGFLP1,MGC126891 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CD31,HBA1,MGC126895,MGC126897 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
CD31,HBA1,MGC126895,MGC126897蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC101846 [Species物种来源]: Homo sapiens 【 CD31,HBA1,MGC126895,MGC126897蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CD31,HBA1,MGC126895,MGC126897 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CD31,HBA1,MGC126895,MGC126897 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CD31,HBA1,MGC126895,MGC126897 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CD31,HBA1,MGC126895,MGC126897 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CD31,HBA1,MGC126895,MGC126897 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CD31,HBA1,MGC126895,MGC126897 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CD31,HBA1,MGC126895,MGC126897 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
HNRPA1,HNRNPA1,MGC102835 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
HNRPA1,HNRNPA1,MGC102835蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103707,BQ425101 [Species物种来源]: Homo sapiens 【 HNRPA1,HNRNPA1,MGC102835蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HNRPA1,HNRNPA1,MGC102835 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HNRPA1,HNRNPA1,MGC102835 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HNRPA1,HNRNPA1,MGC102835 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HNRPA1,HNRNPA1,MGC102835 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens HNRPA1,HNRNPA1,MGC102835 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HNRPA1,HNRNPA1,MGC102835 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HNRPA1,HNRNPA1,MGC102835 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SNX11,MGC111019 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
SNX11,MGC111019蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103721,BU167301 [Species物种来源]: Homo sapiens 【 SNX11,MGC111019蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SNX11,MGC111019 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SNX11,MGC111019 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SNX11,MGC111019 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SNX11,MGC111019 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens SNX11,MGC111019 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SNX11,MGC111019 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SNX11,MGC111019 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
LMN,LMN2,LMNB,LMNB1,MGC111419 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
LMN,LMN2,LMNB,LMNB1,MGC111419蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103723,BU193467 [Species物种来源]: Homo sapiens 【 LMN,LMN2,LMNB,LMNB1,MGC111419蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ LMN,LMN2,LMNB,LMNB1,MGC111419 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 LMN,LMN2,LMNB,LMNB1,MGC111419 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens LMN,LMN2,LMNB,LMNB1,MGC111419 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 LMN,LMN2,LMNB,LMNB1,MGC111419 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens LMN,LMN2,LMNB,LMNB1,MGC111419 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 LMN,LMN2,LMNB,LMNB1,MGC111419 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 LMN,LMN2,LMNB,LMNB1,MGC111419 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
KLC4,KNSL8,MGC111777,BA387M24.3 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
KLC4,KNSL8,MGC111777,BA387M24.3蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103727,BQ437807 [Species物种来源]: Homo sapiens 【 KLC4,KNSL8,MGC111777,BA387M24.3蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ KLC4,KNSL8,MGC111777,BA387M24.3 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 KLC4,KNSL8,MGC111777,BA387M24.3 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens KLC4,KNSL8,MGC111777,BA387M24.3 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 KLC4,KNSL8,MGC111777,BA387M24.3 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens KLC4,KNSL8,MGC111777,BA387M24.3 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 KLC4,KNSL8,MGC111777,BA387M24.3 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 KLC4,KNSL8,MGC111777,BA387M24.3 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
CNIH,CNIL,CNIH1,TGAM77,MGC117156 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
CNIH,CNIL,CNIH1,TGAM77,MGC117156蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103741,BQ233425 [Species物种来源]: Homo sapiens 【 CNIH,CNIL,CNIH1,TGAM77,MGC117156蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CNIH,CNIL,CNIH1,TGAM77,MGC117156 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CNIH,CNIL,CNIH1,TGAM77,MGC117156 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CNIH,CNIL,CNIH1,TGAM77,MGC117156 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CNIH,CNIL,CNIH1,TGAM77,MGC117156 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CNIH,CNIL,CNIH1,TGAM77,MGC117156 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CNIH,CNIL,CNIH1,TGAM77,MGC117156 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CNIH,CNIL,CNIH1,TGAM77,MGC117156 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103743,BQ428213 [Species物种来源]: Homo sapiens 【 H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 H2AZ,H2A.Z,H2A/Z,H2AFZ,MGC117173 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
ZNF573,KIAA0174,MGC117220 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
ZNF573,KIAA0174,MGC117220蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103745,BU169760 [Species物种来源]: Homo sapiens 【 ZNF573,KIAA0174,MGC117220蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ZNF573,KIAA0174,MGC117220 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ZNF573,KIAA0174,MGC117220 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ZNF573,KIAA0174,MGC117220 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ZNF573,KIAA0174,MGC117220 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens ZNF573,KIAA0174,MGC117220 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ZNF573,KIAA0174,MGC117220 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ZNF573,KIAA0174,MGC117220 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103746,BU509393 [Species物种来源]: Homo sapiens 【 M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 M40,TUBB,TUBB1,TUBB5,MGC16435,MGC117247,OK/SW-CL.56 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SMAP,C11ORF58,MGC117265,IMAGE145052蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103747,BU177174 [Species物种来源]: Homo sapiens 【 SMAP,C11ORF58,MGC117265,IMAGE145052蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SMAP,C11ORF58,MGC117265,IMAGE145052 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SMAP,C11ORF58,MGC117265,IMAGE145052 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SMAP,C11ORF58,MGC117265,IMAGE145052 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SMAP,C11ORF58,MGC117265,IMAGE145052 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens SMAP,C11ORF58,MGC117265,IMAGE145052 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SMAP,C11ORF58,MGC117265,IMAGE145052 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SMAP,C11ORF58,MGC117265,IMAGE145052 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
FLJ37478,MGC117272 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
FLJ37478,MGC117272蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103748,BQ882031 [Species物种来源]: Homo sapiens 【 FLJ37478,MGC117272蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FLJ37478,MGC117272 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FLJ37478,MGC117272 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FLJ37478,MGC117272 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FLJ37478,MGC117272 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens FLJ37478,MGC117272 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FLJ37478,MGC117272 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FLJ37478,MGC117272 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
TWIST2 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
TWIST2蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103755,BQ222794 [Species物种来源]: Homo sapiens 【 TWIST2蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TWIST2 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TWIST2 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TWIST2 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TWIST2 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens TWIST2 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TWIST2 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TWIST2 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
SH3BGR,SH3BGRL,MGC117402 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
SH3BGR,SH3BGRL,MGC117402蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103762,BQ880737 [Species物种来源]: Homo sapiens 【 SH3BGR,SH3BGRL,MGC117402蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ SH3BGR,SH3BGRL,MGC117402 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 SH3BGR,SH3BGRL,MGC117402 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens SH3BGR,SH3BGRL,MGC117402 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 SH3BGR,SH3BGRL,MGC117402 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens SH3BGR,SH3BGRL,MGC117402 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 SH3BGR,SH3BGRL,MGC117402 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 SH3BGR,SH3BGRL,MGC117402 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
DCK,MGC117410 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心
DCK,MGC117410蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC103764,BQ946723 [Species物种来源]: Homo sapiens 【 DCK,MGC117410蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ DCK,MGC117410 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 DCK,MGC117410 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens DCK,MGC117410 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 DCK,MGC117410 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens DCK,MGC117410 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 DCK,MGC117410 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 DCK,MGC117410 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
NFAT2,NFATC,NF-ATC,NFATC1,MGC138448蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC104753 [Species物种来源]: Homo sapiens 【 NFAT2,NFATC,NF-ATC,NFATC1,MGC138448蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NFAT2,NFATC,NF-ATC,NFATC1,MGC138448 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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