C7ORF38 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

C7ORF38蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040214,BC040214.1,BI828803,BI828803.1 [Species物种来源]: Homo sapiens 【 C7ORF38蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ C7ORF38 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 C7ORF38 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens C7ORF38 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 C7ORF38 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens C7ORF38 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 C7ORF38 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 C7ORF38 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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TENR,FLJ32741 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

TENR,FLJ32741蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040229,BC040229.1,BI830301,BI830301.1 [Species物种来源]: Homo sapiens 【 TENR,FLJ32741蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ TENR,FLJ32741 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 TENR,FLJ32741 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens TENR,FLJ32741 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 TENR,FLJ32741 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens TENR,FLJ32741 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 TENR,FLJ32741 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 TENR,FLJ32741 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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LOC128439 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

LOC128439蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040237,BC040237.1,BG703635,BG703635.1 [Species物种来源]: Homo sapiens 【 LOC128439蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ LOC128439 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 LOC128439 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens LOC128439 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 LOC128439 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens LOC128439 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 LOC128439 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 LOC128439 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040240,BC040240.2,BE535234,BE535234.1 [Species物种来源]: Homo sapiens 【 MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 MRPL42,MRPS32,PTD007,RPML31,HSPC204,MRP-L31 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040259,BC040259.1,BI754657,BI754657.1 [Species物种来源]: Homo sapiens 【 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 4.1N,EPB41L1,KIAA0338,MGC11072,DKFZP686H17242 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

WDR63,FLJ30067,NYD-SP29,RP11-507C22.2蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040265,BC040265.1,BI828034,BI828034.1 [Species物种来源]: Homo sapiens 【 WDR63,FLJ30067,NYD-SP29,RP11-507C22.2蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 WDR63,FLJ30067,NYD-SP29,RP11-507C22.2 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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HSD9,CCDC83,FLJ42119,MGC34732 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

HSD9,CCDC83,FLJ42119,MGC34732蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040280,BC040280.1,BI832211,BI832211.1 [Species物种来源]: Homo sapiens 【 HSD9,CCDC83,FLJ42119,MGC34732蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ HSD9,CCDC83,FLJ42119,MGC34732 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 HSD9,CCDC83,FLJ42119,MGC34732 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens HSD9,CCDC83,FLJ42119,MGC34732 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 HSD9,CCDC83,FLJ42119,MGC34732 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens HSD9,CCDC83,FLJ42119,MGC34732 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 HSD9,CCDC83,FLJ42119,MGC34732 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 HSD9,CCDC83,FLJ42119,MGC34732 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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FAM116A,FLJ34969 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

FAM116A,FLJ34969蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040291,BC040291.1,BG719319,BG719319.1 [Species物种来源]: Homo sapiens 【 FAM116A,FLJ34969蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FAM116A,FLJ34969 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FAM116A,FLJ34969 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FAM116A,FLJ34969 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FAM116A,FLJ34969 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens FAM116A,FLJ34969 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FAM116A,FLJ34969 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FAM116A,FLJ34969 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

 Biovector

PASD1 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

PASD1蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040301,BC040301.1,BG723594,BG723594.1 [Species物种来源]: Homo sapiens 【 PASD1蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ PASD1 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 PASD1 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens PASD1 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 PASD1 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens PASD1 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 PASD1 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 PASD1 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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NOD1,CARD4 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

NOD1,CARD4蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC040339,BC040339.1,BF794113,BF794113.1 [Species物种来源]: Homo sapiens 【 NOD1,CARD4蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NOD1,CARD4 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NOD1,CARD4 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NOD1,CARD4 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NOD1,CARD4 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens NOD1,CARD4 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NOD1,CARD4 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NOD1,CARD4 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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