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基因组工程共 8114 条

¥17930

2017-02-13

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pGuide-it-ZsGreen1慢病毒CRISPR/Cas9载体荧光报告载体

载体名称:pGuide-it-ZsGreen1质粒类型:慢病毒载体;CRISPR/Cas9载体;荧光报告载体高拷贝/低拷贝:高拷贝克隆方法:限制性内切酶,多克隆位点...

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¥4930

2017-02-22

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eSpCas9(1.1)防脱靶CRISPR-Cas9质粒载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

eSpCas9(1.1) (Plasmid 71814) -BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 PURPOSE Expresses high specificity SpCas9. Px330-like plasmid. eSpCas9(1.1)防脱靶CRISPR-Cas9质粒载体,高精确性精准靶向,三位点突变Mutation K848A, K1003A, & R1060A,CBh启动子,3xFlag标签。Expresses high specificity SpCas9. Px330-like plasmid. Vector backbonepX330-U6-Chimeric_BB-CBh-hSpCas9 (Search Vector Database) Backbone manufacturerFeng Zhang lab (Addgene plasmid #42230) Total vector size (bp)8506 Vector typeMammalian Expression, CRISPR GROWTH IN BACTERIA Bacterial Resistance(s)Ampicillin Growth Temperature37°C Growth Strain(s)Stbl3 Copy numberHigh Copy GENE/INSERT Gene/Insert nameenhanced specificity Cas9 (1.1) Alt nameeSpCas9(1.1) SpeciesS. pyogenes MutationK848A, K1003A, & R1060A PromoterCBh Tag / Fusion Protein 3xFLAG (N terminal on insert) CLONING INFORMATION Cloning methodRestriction Enzyme 5′ cloning siteAgeI (not destroyed) 3′ cloning siteEcoRI (not destroyed) 5′ sequencing primeragggatggttggttggtggg 3′ sequencing primerCCAATCCTCCCCCTTGCTGT >eSpCas9(1.1) sequence 8506 bps gagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaa ttaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggta gtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgat ttcttggctttatatatcttgtggaaaggacgaaacaccgggtcttcgagaagacctgttttagagctag aaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttg ttttagagctagaaatagcaagttaaaataaggctagtccgtttttagcgcgtgcgccaattctgcagac aaatggctctagaggtacccgttacataacttacggtaaatggcccgcctggctgaccgcccaacgaccc ccgcccattgacgtcaatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacgg taaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacg gtaaatggcccgcctggcattgtgcccagtacatgaccttatgggactttcctacttggcagtacatcta cgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctccccc ccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcgggggggg ggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggc ggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctat aaaaagcgaagcgcgcggcgggcgggagtcgctgcgacgctgccttcgccccgtgccccgctccgccgcc gcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggccctt ctcctccgggctgtaattagctgagcaagaggtaagggtttaagggatggttggttggtggggtattaat gtttaattacctggagcacctgcctgaaatcactttttttcaggttggaccggtgccaccATGGACTATA AGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAA GAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATC GGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGC TGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAAC AGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTAT CTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCT TCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTA CCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTG CGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGA ACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGA AAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGG CTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGA GCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAA GGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTG GCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGG CCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCT CGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGC TACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACG GCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGG CAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCA TTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTC TGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTT CGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAAC CTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGA CCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCAT CGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAA ATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACC ACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGA TATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCAC CTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGA AGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTT CGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCC CAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGA AGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAA CATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATG AAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCC AGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACT GGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGGCcGACGACTCC ATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGG TCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGA CAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTG GTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACG AGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAA GGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCC GTCGTGGGAACCGCCCTGATCAAAAAGTACCCTGCGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGG TGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTT CTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGGCGCCT CTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGA AAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGA GTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTAC GGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCA AGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCC CATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTAC TCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACG AACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTC CCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAG CAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACA ACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCT GGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAG GTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGC TGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGTAAgaattcct agagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgc cttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattg tctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagag aatagcaggcatgctggggagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcg ctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagt gagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggt atttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgt ggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttccct tcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgat ttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgcc ctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaact ggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctatt ggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttt atggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacaccc gctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccggga gctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtg cgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccct gataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcc cttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaa gatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttc gccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtat tgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcacca gtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtg ataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaa catgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgag cgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactc tagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggc ccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcattgca gcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatgg atgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagt ttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctt tttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaa agatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccacc gctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagc agagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtag caccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtct taccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgc acacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcg ccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcac gagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgag cgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttac ggttcctggccttttgctggccttttgctcacatgt ​

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2017-02-24

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pY017 (pcDNA3.1-hPcCpf1)质粒CRISPR/Cas9系统载体Plasmid 69989

pY017 (pcDNA3.1-hPcCpf1)(Plasmid 69989)PURPOSEExpresses humanized PcCpf1Vector backbonepcDNA3.1 (Search Vector Database)Ba...

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2017-02-24

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pY018 (pcDNA3.1-hPdCpf1) CRISPR/Cas9载体质粒Plasmid 69990

pY018 (pcDNA3.1-hPdCpf1)(Plasmid 69990)PURPOSEExpresses humanized PdCpf1Vector backbonepcDNA3.1 (Search Vector Database)Ba...

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2017-02-24

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pY019 (pcDNA3.1-hPmCpf1) CRISPR/Cas9载体质粒Plasmid 69991

pY019 (pcDNA3.1-hPmCpf1)(Plasmid 69991)PURPOSEExpresses humanized PmCpf1Vector backbonepcDNA3.1 (Search Vector Database)Ba...

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¥4950

2017-02-24

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CRISPRmTmG2 质粒CRISPR/Cas9系统载体Plasmid 69992

CRISPRmTmG2(Plasmid 69992)Map:PURPOSEThe CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-m...

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¥4930

2017-02-27

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SP-dCas9-VPR基因编辑CRISPR/Cas9系统质粒携带 VP64-p65-Rta,Plasmid 63798

SP-dCas9-VPR (Plasmid 63798)PURPOSESP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vectorCOMME...

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¥27930

2017-03-01

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pTRE-GFP-hCas9四环素诱导型绿色荧光标记基因编辑系统载体CRISPR/Cas9系统-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pTRE-GFP-hCas9四环素诱导型绿色荧光标记基因编辑系统载体CRISPR/Cas9系统描述:带有TRE3G启动子+EGFP绿色荧光+IRES双顺反子+hCas9基因,3x...

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2017-03-02

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA腺相关病毒载体CRISPR/Cas9基因编辑系统-BioVector

pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNAPlasmid 61591PURPOSEA single vector AAV-Cas9 system containing Cas9...

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2017-03-02

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PX552腺相关病毒CRISPR/Cas9基因编辑载体Plasmid 60958-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

PX552(BioVector-060958)Map图谱:PURPOSEpAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA clonin...

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2017-03-02

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pX551腺相关病毒CRISPR/Cas9基因编辑系统载体mecp2启动子-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

PX551(Plasmid 60957)-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心pX551腺相关病毒CRISPR/Cas9基因编辑系统载体,mecp2启动子pA...

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¥19532

2017-04-21

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CRISPR-AsCpf1 plasmid细胞基因编辑系统质粒载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

CRISPR-AsCpf1 plasmid细胞基因编辑系统质粒载体BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.netMap图谱

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2017-04-24

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PX462 pSpCas9n(BB)-T2A-Puro基因编辑CRISPR/Cas9系统载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

载体名称:PX462;pSpCas9n(BB)-T2A-Puro质粒类型:CRISPR/Cas9载体;基因敲除载体;哺乳动物载体高拷贝/低拷贝:高拷贝克隆方法:AgeI,EcoRI启...

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¥29832

2017-05-24

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pcDNA3.1-hAsCpf1-crRNA BioVector NTCC质粒载体菌种细胞基因保藏中心

pcDNA3.1-hAsCpf1-crRNA BioVector NTCC质粒载体菌种细胞基因保藏中心 Expresses human codon-optimized AsCpf1...

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¥7930

2017-05-24

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BPK3082 gRNA vector for LbCpf1 expression plasmid SQT1665 BioVector NTCC质粒载体菌种细胞基因保藏中心

BPK3082 gRNA vector for LbCpf1 expression plasmid SQT1665 gRNA vector for LbCpf1 expression plasmid SQT1665. ...

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2017-05-24

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pAsCpf1-gRNA基因编辑CRISPR-Cpf1系统guide RNA克隆载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAsCpf1-gRNA pAsCpf1-gRNA基因编辑CRISPR-Cpf1系统guide RNA克隆载体,将AsCpf1 crRNA spacer序列插入到BsmBI位点之间,U6启动子,Amp...

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¥9852

2017-08-18

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gRNA_Cloning Vector 基因编辑CRISPR/Cas9系统载体-BioVector NTCC Inc.

质粒名称:gRNA_Cloning Vector 目录编号:41824存储实验室:George Church用途:一个空的gRNA表达载体,用于创建一个特定序列的基因组。载...

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¥17935

2017-09-08

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pX330-dCas9-gRNA基因编辑CRISPR-Cas9载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pX330-dCas9-gRNA基因编辑CRISPR-Cas9载体表达人源优化的dCas9基因和gRNA序列,CAG启动子,3xFlag标签,NLS核定位信号,U6启动子+gRNA,Amp...

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¥28925

2017-09-25

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DUBs文库 BioVector NTCC质粒载体菌种细胞基因保藏中心

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¥49735

2017-09-25

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激酶基因文库 BioVector NTCC质粒载体菌种细胞基因保藏中心

激酶基因文库 BioVector NTCC质粒载体菌种细胞基因保藏中心 [Supplier来源] http://www.biovector.net

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